Overview
- External ontology
- TOGO:M1848 — Methanobacterium alcaliphilum medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1848 Original source: NBRC - NBRC_M1083 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1083
Composition
flowchart LR
CultureMech_008422["methanobacterium_alcaliphilum_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008422 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008422 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_NH4Cl["NH4Cl"]
CultureMech_008422 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_K2HPO4["K2HPO4"]
CultureMech_008422 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Resazurin["Resazurin"]
CultureMech_008422 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008422 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_Na2S_9H2O["Na2S·9H2O"]
CultureMech_008422 --> ing6_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_9H2O -.-> CHEBI_76209
ing7_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008422 --> ing7_Bacto_Yeast_Extract__Difco_
ing8_Cysteine_HCl["Cysteine-HCl"]
CultureMech_008422 --> ing8_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing8_Cysteine_HCl -.-> CHEBI_91247
ing9_Polypeptone["Polypeptone"]
CultureMech_008422 --> ing9_Polypeptone
ing10_N2["N2"]
CultureMech_008422 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
ing11_H2["H2"]
CultureMech_008422 --> ing11_H2
CHEBI_18276["CHEBI:18276"]
ing11_H2 -.-> CHEBI_18276
ing12_NaHCO3["NaHCO3"]
CultureMech_008422 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
ing13_Na2CO3["Na2CO3"]
CultureMech_008422 --> ing13_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing13_Na2CO3 -.-> CHEBI_29377
ing14_Tris["Tris"]
CultureMech_008422 --> ing14_Tris
CHEBI_9754["CHEBI:9754"]
ing14_Tris -.-> CHEBI_9754
ing15_HCl["HCl"]
CultureMech_008422 --> ing15_HCl
CHEBI_17883["CHEBI:17883"]
ing15_HCl -.-> CHEBI_17883
ing16_Biotin["Biotin"]
CultureMech_008422 --> ing16_Biotin
CHEBI_15956["CHEBI:15956"]
ing16_Biotin -.-> CHEBI_15956
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008422 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18_Thiamine_HCl["Thiamine-HCl"]
CultureMech_008422 --> ing18_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing18_Thiamine_HCl -.-> CHEBI_49105
ing19_Ca_pantothenate["Ca-pantothenate"]
CultureMech_008422 --> ing19_Ca_pantothenate
CHEBI_29032["CHEBI:29032"]
ing19_Ca_pantothenate -.-> CHEBI_29032
ing20_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_008422 --> ing20_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing20_Pyridoxine_HCl -.-> CHEBI_30961
ing21_Folic_acid["Folic acid"]
CultureMech_008422 --> ing21_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing21_Folic_acid -.-> CHEBI_27470
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_008422 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_Riboflavin["Riboflavin"]
CultureMech_008422 --> ing23_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing23_Riboflavin -.-> CHEBI_17015
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_008422 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008422 --> ing25_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing25_MgSO4_7H2O -.-> CHEBI_31795
ing26_NaCl["NaCl"]
CultureMech_008422 --> ing26_NaCl
CHEBI_26710["CHEBI:26710"]
ing26_NaCl -.-> CHEBI_26710
ing27_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008422 --> ing27_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing27_Na2MoO4_2H2O -.-> CHEBI_75213
ing28_H3BO3["H3BO3"]
CultureMech_008422 --> ing28_H3BO3
CHEBI_33118["CHEBI:33118"]
ing28_H3BO3 -.-> CHEBI_33118
ing29_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008422 --> ing29_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing29_FeSO4_7H2O -.-> CHEBI_75836
CultureMech_008422 --> more["...9 more"]
Ingredients (39)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
972.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 760.0, 210.0, 1.0, 1.0] |
| 2 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.23 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.1, 0.13] |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 6 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Bacto Yeast Extract (Difco) | 2 G_PER_L | Properties: Undefined component | |
| 9 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.3 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
Properties: Organic compound, Simple component, Defined component |
| 10 | Polypeptone | 2 G_PER_L | Properties: Undefined component | |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 |
H2
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Tris
C4H11NO3 121.136 g/mol
|
variable VARIABLE |
CHEBI:9754
tris
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 16 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
| 17 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
1 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
5 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.011 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.17 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 34 |
KAl(SO4)2·12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.018 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 35 |
MnSO4·4H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.55 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 36 |
Na2WO4
2Na.O4W 293.816 g/mol
|
0.004 G_PER_L |
CHEBI:63940
sodium tungstate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 37 |
CuSO4
Cu.O4S 159.609 g/mol
|
0.05 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 38 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.004 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 39 |
2Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.64 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Simple component, Organic compound |
Solutions
Tris-HCl buffer**
Dose: 10 G_PER_L
Role: Buffer; Properties: Defined component, Complex component, Solution
Carbonate mixture*
Dose: 210 G_PER_L
Vitamin solution***
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Trace elements solution****
Dose: 10 G_PER_L
See mediadive_6129_Trace_elements_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1848_Methanobacterium_alcaliphilum_medium (CultureMech:008422)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.501180Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1848 |
| 2026-01-28T19:48:24.293681Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: H2; Added default concentration for ingredient: N2; Added default concentration for ingredient: Tris; Added default concentration for ingredient: HCl; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:40.158396+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:10.098073Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:19:56.721505Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008422 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.290080+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanobacterium alcaliphilum medium" to "methanobacterium_alcaliphilum_medium" for consistent matching |
| 2026-05-16T09:03:11.482281+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:34.605482+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:31.192908+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:24.703906+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.289126+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:25.806407+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b007e50745c7fc502552fd4e93bf034400669447f2fec2cc33345ea98e66ae5a |