Overview
- External ontology
- TOGO:M1856 — Modified NBRC medium 802 (pH 10, 0.6 M total Na+)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1856 Original source: NBRC - NBRC_M1099 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1099
Composition
flowchart LR
CultureMech_008430["modified_nbrc_medium_802_ph_10_0_6_m_total_na"]
ing0_Distilled_water["Distilled water"]
CultureMech_008430 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008430 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008430 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_008430 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Agar__if_needed_["Agar (if needed)"]
CultureMech_008430 --> ing4_Agar__if_needed_
ing5_NaCl["NaCl"]
CultureMech_008430 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_NaHCO3["NaHCO3"]
CultureMech_008430 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Na2CO3["Na2CO3"]
CultureMech_008430 --> ing7_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing7_Na2CO3 -.-> CHEBI_29377
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 900.0, 100.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
variable VARIABLE |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
variable VARIABLE |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 8 |
Na2CO3
CO3.2Na 105.988 g/mol
|
variable VARIABLE |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
Solutions
Hipolypepton*
Dose: 10 G_PER_L
Properties: Undefined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_nbrc_medium_802_ph_10_0_6_m_total_na (CultureMech:008430)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.518012Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1856 |
| 2026-01-28T19:48:35.789563Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaCl; Added default concentration for ingredient: K2HPO4; Added default concentration for ingredient: NaHCO3; Added default concentration for ingredient: Na2CO3 |
| 2026-03-09T08:04:59.594673+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:31.344402Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:56.791412Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008430 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.364072+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified NBRC medium 802 (pH 10, 0.6 M total Na+)" to "modified_nbrc_medium_802_ph_10_0_6_m_total_na" for consistent matching |
| 2026-05-16T09:04:26.039989+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:37.486829+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:26.594102+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.870045+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:49.258946+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 03bec067ecacd4bb940eeda97e7c8b66644f3eec2c59e353696b58d788b108f6 |