Overview
- External ontology
- TOGO:M1863 — Medium for strains JA430
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'medium_for_strain_ja349_and_ja350', 'source': 'TOGO', 'source_id': 'TOGO:M1827', 'original_category': 'bacterial'}
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1863 Original source: NBRC - NBRC_M1107 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1107
Composition
flowchart LR
CultureMech_008438["medium_for_strains_ja430"]
ing0_Vitamin_B12__0_002___w_v__["Vitamin B12 (0.002% (w/v))"]
CultureMech_008438 --> ing0_Vitamin_B12__0_002___w_v__
ing1_NaHCO3__10___w_v__["NaHCO3 (10% (w/v))"]
CultureMech_008438 --> ing1_NaHCO3__10___w_v__
ing2_Distilled_water["Distilled water"]
CultureMech_008438 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008438 --> ing3_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_7H2O -.-> CHEBI_31795
ing4_Yeast_extract["Yeast extract"]
CultureMech_008438 --> ing4_Yeast_extract
ing5_NaCl["NaCl"]
CultureMech_008438 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008438 --> ing6_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_2H2O -.-> CHEBI_86158
ing7_KH2PO4["KH2PO4"]
CultureMech_008438 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_NH4Cl["NH4Cl"]
CultureMech_008438 --> ing8_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing8_NH4Cl -.-> CHEBI_31206
ing9_Resazurin["Resazurin"]
CultureMech_008438 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_Na2S_9H2O["Na2S·9H2O"]
CultureMech_008438 --> ing10_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_9H2O -.-> CHEBI_76209
ing11_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008438 --> ing11_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing11_Sodium_pyruvate -.-> CHEBI_50144
ing12_N2["N2"]
CultureMech_008438 --> ing12_N2
CHEBI_17997["CHEBI:17997"]
ing12_N2 -.-> CHEBI_17997
ing13_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008438 --> ing13_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_2H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_008438 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008438 --> ing15_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_4H2O -.-> CHEBI_86368
ing16_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008438 --> ing16_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing16_CoCl2_6H2O -.-> CHEBI_35696
ing17_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008438 --> ing17_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_6H2O -.-> CHEBI_34887
ing18_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008438 --> ing18_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_2H2O -.-> CHEBI_86318
ing19_ZnCl2["ZnCl2"]
CultureMech_008438 --> ing19_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing19_ZnCl2 -.-> CHEBI_49976
ing20_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
CultureMech_008438 --> ing20_HCl__25___7_7_M_
ing21_FeCl2_4H2O["FeCl2·4H2O"]
CultureMech_008438 --> ing21_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing21_FeCl2_4H2O -.-> CHEBI_86249
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Vitamin B12 (0.002% (w/v)) | 1 G_PER_L | Role: Vitamin; Properties: Defined component, Organic compound, Solution | |
| 2 | NaHCO3 (10% (w/v)) | 15 G_PER_L | Role: Buffer; Properties: Solution, Inorganic compound, Defined component | |
| 3 |
Distilled water
H2O 18.015 g/mol
|
991.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 990.0] |
| 4 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.68 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 11 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
0.24 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 13 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 14 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
40 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
60 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
200 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
20 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
20 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 | HCl (25%; 7.7 M) | 10 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component | |
| 22 |
FeCl2·4H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Ferric citrate solution* (0.1%)
Dose: 5 G_PER_L
Role: Mineral source; Properties: Defined component, Organic compound, Solution
Trace element solution SL7*
Dose: 1 G_PER_L
See mediadive_2739_Trace_element_solution_SL7.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- medium_for_strain_ja349_and_ja350 (CultureMech:008399)
- medium_for_strains_ja430 (CultureMech:008438)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.534742Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1863 |
| 2026-01-28T19:48:19.002632Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:31.468883+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:00.577517Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:56.876190Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008438 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.452185+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for strains JA430" to "medium_for_strains_ja430" for consistent matching |
| 2026-05-16T09:04:20.861034+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:29.988495+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:21.712038+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:06.402146+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:43.548399+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: medium_for_strain_ja349_and_ja350, medium_for_strains_ja430 |
| 2026-08-06T03:05:43.548457+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint c2ec1137a5f0e2b265b2abf80234735df7d34d3f5cd5e9e15405ddcd3972aeca |