Overview
- External ontology
- TOGO:M1868 — Sporulation Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1868 Original source: NBRC - NBRC_M1113 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1113
Composition
flowchart LR
CultureMech_008442["sporulation_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008442 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008442 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008442 --> ing2_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing2_FeSO4_7H2O -.-> CHEBI_75836
ing3_KCl["KCl"]
CultureMech_008442 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Agar__if_needed_["Agar (if needed)"]
CultureMech_008442 --> ing4_Agar__if_needed_
ing5_Nutrient_broth["Nutrient broth"]
CultureMech_008442 --> ing5_Nutrient_broth
ing6_Ca_NO3_2_4H2O["Ca(NO3)2·4H2O"]
CultureMech_008442 --> ing6_Ca_NO3_2_4H2O
CHEBI_86159["CHEBI:86159"]
ing6_Ca_NO3_2_4H2O -.-> CHEBI_86159
ing7_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008442 --> ing7_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_4H2O -.-> CHEBI_86368
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Defined component, Inorganic compound [Merged 3 duplicates: 800.0, 100.0, 100.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.278 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 6 | Nutrient broth | 8 G_PER_L | Properties: Undefined component, Complex component | |
| 7 |
Ca(NO3)2·4H2O
Ca.4H2O.2NO3 236.146 g/mol
|
236.15 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.979 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1868_Sporulation_Medium (CultureMech:008442)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.546074Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1868 |
| 2026-01-28T19:48:17.696550Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:58.157641Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:56.921579Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008442 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.502649+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sporulation Medium" to "sporulation_medium" for consistent matching |
| 2026-06-05T07:40:34.653099+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:24.749032+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:09.817450+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5840b1986492e1c56b04f298cfb5b2e4cae9701fc36d5fde4559b852b0f53f0e |