Overview
- External ontology
- TOGO:M1878 — MMB medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1878 Original source: NBRC - NBRC_M1123 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1123
Composition
flowchart LR
CultureMech_008452["mmb_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008452 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008452 --> ing1_Yeast_extract
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_008452 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_Glucose["Glucose"]
CultureMech_008452 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Peptone["Peptone"]
CultureMech_008452 --> ing4_Peptone
ing5_Biotin["Biotin"]
CultureMech_008452 --> ing5_Biotin
CHEBI_15956["CHEBI:15956"]
ing5_Biotin -.-> CHEBI_15956
ing6_Thiamine_HCl["Thiamine-HCl"]
CultureMech_008452 --> ing6_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing6_Thiamine_HCl -.-> CHEBI_49105
ing7_Ca_pantothenate["Ca-pantothenate"]
CultureMech_008452 --> ing7_Ca_pantothenate
CHEBI_29032["CHEBI:29032"]
ing7_Ca_pantothenate -.-> CHEBI_29032
ing8_Folic_acid["Folic acid"]
CultureMech_008452 --> ing8_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing8_Folic_acid -.-> CHEBI_27470
ing9_Vitamin_B12["Vitamin B12"]
CultureMech_008452 --> ing9_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing9_Vitamin_B12 -.-> CHEBI_176843
ing10_Riboflavin["Riboflavin"]
CultureMech_008452 --> ing10_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing10_Riboflavin -.-> CHEBI_17015
ing11_Nicotinamide["Nicotinamide"]
CultureMech_008452 --> ing11_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing11_Nicotinamide -.-> CHEBI_17154
ing12_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008452 --> ing12_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_7H2O -.-> CHEBI_31795
ing13_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008452 --> ing13_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_2H2O -.-> CHEBI_86158
ing14_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008452 --> ing14_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_7H2O -.-> CHEBI_75836
ing15_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
CultureMech_008452 --> ing15_Nitrilotriacetic_acid__NTA_
CHEBI_44557["CHEBI:44557"]
ing15_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
ing16__NH4_6MoO7O24_4H2O["(NH4)6MoO7O24·4H2O"]
CultureMech_008452 --> ing16__NH4_6MoO7O24_4H2O
ing17_KOH["KOH"]
CultureMech_008452 --> ing17_KOH
CHEBI_32035["CHEBI:32035"]
ing17_KOH -.-> CHEBI_32035
ing18_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008452 --> ing18_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_7H2O -.-> CHEBI_32312
ing19_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008452 --> ing19_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4_5H2O -.-> CHEBI_31440
ing20_MnSO4_H2O["MnSO4·H2O"]
CultureMech_008452 --> ing20_MnSO4_H2O
CHEBI_86364["CHEBI:86364"]
ing20_MnSO4_H2O -.-> CHEBI_86364
ing21_Co_NO3_2_6H2O["Co(NO3)2·6H2O"]
CultureMech_008452 --> ing21_Co_NO3_2_6H2O
CHEBI_86214["CHEBI:86214"]
ing21_Co_NO3_2_6H2O -.-> CHEBI_86214
ing22_Na2B4O7_10H2O["Na2B4O7·10H2O"]
CultureMech_008452 --> ing22_Na2B4O7_10H2O
CHEBI_131366["CHEBI:131366"]
ing22_Na2B4O7_10H2O -.-> CHEBI_131366
ing23_Na_EDTA["Na-EDTA"]
CultureMech_008452 --> ing23_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing23_Na_EDTA -.-> CHEBI_64734
ing24_H2SO4["H2SO4"]
CultureMech_008452 --> ing24_H2SO4
CHEBI_26836["CHEBI:26836"]
ing24_H2SO4 -.-> CHEBI_26836
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
953.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 1.0, 1.0, 950.0, 1.0] |
| 2 | Yeast extract | 0.15 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.25 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 5 | Peptone | 0.15 G_PER_L | ||
| 6 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 7 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 8 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
5 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 9 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 10 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 11 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 12 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
5 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 13 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.34 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
599.0 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 99.0, 500.0] |
| 16 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 17 | (NH4)6MoO7O24·4H2O | 9.25 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 18 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 19 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
1095 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
39.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
MnSO4·H2O
H2O.Mn.O4S 169.016 g/mol
|
154 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Co(NO3)2·6H2O
Co.6H2O.2NO3 291.031 g/mol
|
24.8 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Na2B4O7·10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
17.7 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
250 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 25 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
Solutions
Vitamin solution*
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Hutner's basal salts**
Dose: 20 G_PER_L
Metal salt solution 44***
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1878_MMB_medium (CultureMech:008452)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.565500Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1878 |
| 2026-01-28T19:48:42.283792Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: H2SO4 |
| 2026-03-09T08:05:11.259153+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:43.093949Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:57.021387Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008452 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.609119+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMB medium" to "mmb_medium" for consistent matching |
| 2026-05-16T09:03:11.593259+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:34.722164+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:24.817930+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.332392+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:09.903860+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3242a11302d5d536cf754389d69d16514a41bb6946f1b4619cdf60f5785f345d |