Overview
- External ontology
- TOGO:M187 — Mmys-II Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M187 Original source: JCM - JCM_M194 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=194
Composition
flowchart LR
CultureMech_008454["mmys_ii_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008454 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008454 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008454 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_008454 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_008454 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_008454 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_Na2S2O3_5H2O["Na2S2O3・5H2O"]
CultureMech_008454 --> ing6_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing6_Na2S2O3_5H2O -.-> CHEBI_32150
ing7_Sodium_DL_malate["Sodium DL-malate"]
CultureMech_008454 --> ing7_Sodium_DL_malate
CHEBI_91260["CHEBI:91260"]
ing7_Sodium_DL_malate -.-> CHEBI_91260
ing8_20_mM_Potassium_phosphate_buffer__pH_7_8_["20 mM Potassium phosphate buffer (pH 7.8)"]
CultureMech_008454 --> ing8_20_mM_Potassium_phosphate_buffer__pH_7_8_
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
500 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
8.2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 8 | Sodium DL-malate | 3.6 G_PER_L |
CHEBI:91260
disodium malate
|
Properties: Defined component, Simple component, Organic compound |
| 9 | 20 mM Potassium phosphate buffer (pH 7.8) | 500 G_PER_L | Role: Buffer; Properties: Defined component, Solution, Inorganic compound, Complex component |
Solutions
Solution I
Dose: 500 G_PER_L
See mediadive_5335_Solution_I.yaml for composition
Solution II
Dose: 500 G_PER_L
See mediadive_5336_Solution_II.yaml for composition
Trace element solution SL8 (see Medium [M183])
Dose: 1 G_PER_L
Cross-reference to Medium M183
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M187_Mmys-II_Medium (CultureMech:008454)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.575288Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M187 |
| 2026-01-28T19:48:21.293658Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:35.498255+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:57.045615Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008454 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.634842+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mmys-II Medium" to "mmys_ii_medium" for consistent matching |
| 2026-05-16T09:03:11.620986+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:34.750086+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.346134+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:09.936101+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0c1762f3960fdd9691f8a78ddeb874142dcce5e3ede99cb0d50c1806a22473cd |