mineral_salts_medium

CultureMech:008457 bacterial COMPLEX SOLID_AGAR
Original name: Mineral salts medium

Overview

External ontology
TOGO:M1883 — Mineral salts medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1883 Original source: NBRC - NBRC_M1127 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1127

Composition

flowchart LR
    CultureMech_008457["mineral_salts_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008457 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008457 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008457 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_K2HPO4["K2HPO4"]
    CultureMech_008457 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_008457 --> ing4_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_7H2O -.-> CHEBI_75836
    ing5_NaNO3["NaNO3"]
    CultureMech_008457 --> ing5_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing5_NaNO3 -.-> CHEBI_63005
    ing6_MnSO4_5H2O["MnSO4·5H2O"]
    CultureMech_008457 --> ing6_MnSO4_5H2O
    CHEBI_131524["CHEBI:131524"]
    ing6_MnSO4_5H2O -.-> CHEBI_131524
    ing7_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008457 --> ing7_Agar__if_needed_

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
3 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 K2HPO4
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
5 FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
0.02 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6 NaNO3
NO3.Na 84.994 g/mol
2 G_PER_L CHEBI:63005
sodium nitrate
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
7 MnSO4·5H2O
5H2O.Mn.O4S 241.076 g/mol
0.02 G_PER_L CHEBI:131524
manganese(II) sulfate pentahydrate
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8 Agar (if needed) 15 G_PER_L Role: Solidifying component

Solutions

Glucose*

Dose: 5 G_PER_L

Role: Carbon source; Properties: Defined component, Organic compound, Simple component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:58.578460Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1883
2026-01-28T19:48:16.722076Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:27.633824+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:57.081539Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008457
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:15.672932+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Mineral salts medium" to "mineral_salts_medium" for consistent matching
2026-05-16T09:04:23.934911+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:34.549554+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:24.619239+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:08.034492+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:46.185076+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint b32f3dbc9361c6915942078d5f437e6072806bba8166ffc8405633894233bd8b