Overview
- External ontology
- TOGO:M1924 — MMJYP2 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1924 Original source: NBRC - NBRC_M1190 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1190
Composition
flowchart LR
CultureMech_008503["mmjyp2_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_008503 --> ing0_Yeast_extract
ing1_NaHCO3["NaHCO3"]
CultureMech_008503 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008503 --> ing2_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing2_Sodium_pyruvate -.-> CHEBI_50144
ing3_Na2S["Na2S"]
CultureMech_008503 --> ing3_Na2S
CHEBI_76208["CHEBI:76208"]
ing3_Na2S -.-> CHEBI_76208
ing4_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
CultureMech_008503 --> ing4_Bacto_Tryptone__Difco_
ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008503 --> ing5_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing5_Carbon_dioxide_gas -.-> CHEBI_16526
ing6_Nitrogen_gas["Nitrogen gas"]
CultureMech_008503 --> ing6_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing6_Nitrogen_gas -.-> CHEBI_17997
ing7_Distilled_water["Distilled water"]
CultureMech_008503 --> ing7_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing7_Distilled_water -.-> CHEBI_15377
ing8_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008503 --> ing8_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_7H2O -.-> CHEBI_31795
ing9_NaCl["NaCl"]
CultureMech_008503 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008503 --> ing10_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_2H2O -.-> CHEBI_86158
ing11_NH4Cl["NH4Cl"]
CultureMech_008503 --> ing11_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing11_NH4Cl -.-> CHEBI_31206
ing12_K2HPO4["K2HPO4"]
CultureMech_008503 --> ing12_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing12_K2HPO4 -.-> CHEBI_131527
ing13_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008503 --> ing13_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing13_MgCl2_6H2O -.-> CHEBI_86345
ing14_KCl["KCl"]
CultureMech_008503 --> ing14_KCl
CHEBI_32588["CHEBI:32588"]
ing14_KCl -.-> CHEBI_32588
ing15_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008503 --> ing15_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_2H2O -.-> CHEBI_75213
ing16_H3BO3["H3BO3"]
CultureMech_008503 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008503 --> ing17_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_7H2O -.-> CHEBI_75836
ing18_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008503 --> ing18_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_6H2O -.-> CHEBI_34887
ing19_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008503 --> ing19_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_7H2O -.-> CHEBI_32312
ing20_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008503 --> ing20_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_5H2O -.-> CHEBI_31440
ing21_Na2SeO3_5H2O["Na2SeO3·5H2O"]
CultureMech_008503 --> ing21_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing21_Na2SeO3_5H2O -.-> CHEBI_131361
ing22_CoSO4_7H2O["CoSO4·7H2O"]
CultureMech_008503 --> ing22_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing22_CoSO4_7H2O -.-> CHEBI_91244
ing23_KAl_SO4_2_12H2O["KAl(SO4)2·12H2O"]
CultureMech_008503 --> ing23_KAl_SO4_2_12H2O
CHEBI_86465["CHEBI:86465"]
ing23_KAl_SO4_2_12H2O -.-> CHEBI_86465
ing24_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
CultureMech_008503 --> ing24_Nitrilotriacetic_acid__NTA_
CHEBI_44557["CHEBI:44557"]
ing24_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
ing25_MnSO4_2H2O["MnSO4·2H2O"]
CultureMech_008503 --> ing25_MnSO4_2H2O
CHEBI_86356["CHEBI:86356"]
ing25_MnSO4_2H2O -.-> CHEBI_86356
ing26_KOH["KOH"]
CultureMech_008503 --> ing26_KOH
CHEBI_32035["CHEBI:32035"]
ing26_KOH -.-> CHEBI_32035
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 4 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 4 |
Na2S
2Na.S 78.047 g/mol
|
0.5 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 5 | Bacto Tryptone (Difco) | 4 G_PER_L | Properties: Undefined component | |
| 6 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 7 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 8 |
Distilled water
H2O 18.015 g/mol
|
991.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 990.0, 1.0] |
| 9 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 3.4, 3.0] |
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
26.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 25.0, 1.0] |
| 11 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.7999999999999999 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.7, 0.1] |
| 12 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2SeO3·5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
CoSO4·7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
KAl(SO4)2·12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 26 |
MnSO4·2H2O
2H2O.Mn.O4S 187.031 g/mol
|
0.5 G_PER_L |
CHEBI:86356
manganese(II) sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
MJ synthetic seawater for MMJYP2 medium *
Dose: 1 G_PER_L
Balch's trace mineral solution**
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mmjyp2_medium (CultureMech:008503)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.673946Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1924 |
| 2026-01-28T19:48:40.330577Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: KOH |
| 2026-03-09T08:05:07.978277+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:39.695845Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:57.560726Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008503 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.176245+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJYP2 Medium" to "mmjyp2_medium" for consistent matching |
| 2026-05-16T09:04:24.700834+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:35.729208+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:25.350479+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.369572+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.485826+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9b94ddc89ded97eaeb717e875b8b67d0ccb316adbd091e16784843505715b96c |