Overview
- External ontology
- TOGO:M1931 — DG18: Dichloran 18% Glycerol Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1931 Original source: NBRC - NBRC_M1198 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1198
Composition
flowchart LR
CultureMech_008510["dg18_dichloran_18_glycerol_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_008510 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008510 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_008510 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Glycerol["Glycerol"]
CultureMech_008510 --> ing3_Glycerol
CHEBI_17754["CHEBI:17754"]
ing3_Glycerol -.-> CHEBI_17754
ing4_Chloramphenicol["Chloramphenicol"]
CultureMech_008510 --> ing4_Chloramphenicol
ing5_Glucose["Glucose"]
CultureMech_008510 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Agar["Agar"]
CultureMech_008510 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Dichloran__0_2__in_ethanol_["Dichloran (0.2% in ethanol)"]
CultureMech_008510 --> ing7_Dichloran__0_2__in_ethanol_
ing8_Peptone["Peptone"]
CultureMech_008510 --> ing8_Peptone
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Glycerol
C3H8O3 92.094 g/mol
|
220 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 5 | Chloramphenicol | 0.1 G_PER_L | Role: Antimicrobial agent; Properties: Simple component, Defined component, Organic compound | |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Dichloran (0.2% in ethanol) | 1 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 9 | Peptone | 5 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1931_DG18_Dichloran_18_Glycerol_Agar (CultureMech:008510)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.692414Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1931 |
| 2026-01-28T19:48:18.971164Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:57.638539Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008510 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.256918+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DG18: Dichloran 18% Glycerol Agar" to "dg18_dichloran_18_glycerol_agar" for consistent matching |
| 2026-06-05T07:40:34.980066+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:31.437809+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:25.035537+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.429643+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:10.047966+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 49c6655a9d4d639a4a8e7230e19958a914e1a0a91c70422ca1fa8bf9071619c0 |