Overview
- External ontology
- TOGO:M1937 — LB + Kanamycin medium
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'lb_50_ug_ml_kanamycin_medium', 'source': 'TOGO', 'source_id': 'TOGO:M2099', 'original_category': 'bacterial'}
- {'name': 'lb_ampicillin_medium', 'source': 'TOGO', 'source_id': 'TOGO:M1685', 'original_category': 'bacterial'}
- Notes
- Source: https://togomedium.org/medium/M1937 Original source: NBRC - NBRC_M1204 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1204
Composition
flowchart LR
CultureMech_008515["lb_kanamycin_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008515 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008515 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008515 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Agar__if_needed_["Agar (if needed)"]
CultureMech_008515 --> ing3_Agar__if_needed_
ing4_Bacto_Tyrptone__Difco_["Bacto Tyrptone (Difco)"]
CultureMech_008515 --> ing4_Bacto_Tyrptone__Difco_
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 5 | Bacto Tyrptone (Difco) | 10 G_PER_L | Properties: Undefined component |
Solutions
Kanamycin sulfate solution (20 mg/ml)*
Dose: 1 G_PER_L
Role: Antimicrobial agent; Properties: Solution, Organic compound, Defined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- lb_50_ug_ml_kanamycin_medium (CultureMech:008689)
- lb_ampicillin_medium (CultureMech:008244)
- lb_kanamycin_medium (CultureMech:008515)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.700125Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1937 |
| 2026-01-28T19:48:32.477356Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:53.900269+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:57.669330Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008515 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.289196+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB + Kanamycin medium" to "lb_kanamycin_medium" for consistent matching |
| 2026-05-16T09:03:38.845109+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:10.874303+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:39.962933+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: lb_50_ug_ml_kanamycin_medium, lb_ampicillin_medium, lb_kanamycin_medium |
| 2026-08-06T03:05:39.963052+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint e971202adc752bdedf4447173841d5e75a9a588ff7de9fcfd3e67e41a3298c26 |