Overview
- External ontology
- TOGO:M1941 — A3
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1941 Original source: NBRC - NBRC_M1210 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1210
Composition
flowchart LR
CultureMech_008520["a3"]
ing0_Distilled_water["Distilled water"]
CultureMech_008520 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008520 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008520 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KH2PO4["KH2PO4"]
CultureMech_008520 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_008520 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KNO3["KNO3"]
CultureMech_008520 --> ing5_KNO3
CHEBI_63043["CHEBI:63043"]
ing5_KNO3 -.-> CHEBI_63043
ing6_CaCl2_H2O["CaCl2·H2O"]
CultureMech_008520 --> ing6_CaCl2_H2O
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2_H2O -.-> CHEBI_3312
ing7_Agar__if_needed_["Agar (if needed)"]
CultureMech_008520 --> ing7_Agar__if_needed_
ing8_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008520 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_H2SO4["H2SO4"]
CultureMech_008520 --> ing9_H2SO4
CHEBI_26836["CHEBI:26836"]
ing9_H2SO4 -.-> CHEBI_26836
ing10_H3BO3["H3BO3"]
CultureMech_008520 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008520 --> ing11_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_7H2O -.-> CHEBI_32312
ing12_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008520 --> ing12_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_5H2O -.-> CHEBI_31440
ing13_MnSO4_7H2O["MnSO4·7H2O"]
CultureMech_008520 --> ing13_MnSO4_7H2O
CHEBI_86360["CHEBI:86360"]
ing13_MnSO4_7H2O -.-> CHEBI_86360
ing14_Na2MoO4["Na2MoO4"]
CultureMech_008520 --> ing14_Na2MoO4
CHEBI_75215["CHEBI:75215"]
ing14_Na2MoO4 -.-> CHEBI_75215
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
502.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 500.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
75 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
25 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
175 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
75 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KNO3
K.NO3 101.102 g/mol
|
250 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
CaCl2·H2O
Ca.2Cl 110.984 g/mol
|
10 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 9 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
2.86 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
222 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
79 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnSO4·7H2O
Mn.O4S 151.001 g/mol
|
2.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
21 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
Solutions
Fe Solution*
Dose: 1 G_PER_L
See mediadive_5640_Fe_solution.yaml for composition
A5 Solution**
Dose: 1 G_PER_L
See mediadive_4987_A5_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- a3 (CultureMech:008520)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.707883Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1941 |
| 2026-01-28T19:48:31.804934Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-09T08:04:52.974571+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:24.410102Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:57.711593Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008520 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.333180+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "A3" to "a3" for consistent matching |
| 2026-05-16T09:03:22.699577+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:46.446300+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:34.533026+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.296612+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:21.130606+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3f563b10e8c84f91878dbc7550a1cbcd903f9d736abae0607c914ce3dbeb0a93 |