medium_for_strain_ja746

CultureMech:008530 bacterial COMPLEX LIQUID
Original name: Medium for Strain JA746

Overview

External ontology
TOGO:M1950 — Medium for Strain JA746
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M1950 Original source: NBRC - NBRC_M1224 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1224

Composition

flowchart LR
    CultureMech_008530["medium_for_strain_ja746"]
    ing0_Vitamin_B12__0_002___w_v__["Vitamin B12 (0.002% (w/v))"]
    CultureMech_008530 --> ing0_Vitamin_B12__0_002___w_v__
    ing1_Distilled_water["Distilled water"]
    CultureMech_008530 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008530 --> ing2_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_7H2O -.-> CHEBI_31795
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_008530 --> ing3_Yeast_extract
    ing4_NaCl["NaCl"]
    CultureMech_008530 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008530 --> ing5_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_2H2O -.-> CHEBI_86158
    ing6_KH2PO4["KH2PO4"]
    CultureMech_008530 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_NH4Cl["NH4Cl"]
    CultureMech_008530 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_Resazurin["Resazurin"]
    CultureMech_008530 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_008530 --> ing9_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_9H2O -.-> CHEBI_76209
    ing10_Sodium_acetate["Sodium acetate"]
    CultureMech_008530 --> ing10_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing10_Sodium_acetate -.-> CHEBI_32954
    ing11_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_008530 --> ing11_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing11_Sodium_pyruvate -.-> CHEBI_50144
    ing12_Sodium_succinate["Sodium succinate"]
    CultureMech_008530 --> ing12_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing12_Sodium_succinate -.-> CHEBI_63675
    ing13_nitrogen_gas["nitrogen gas"]
    CultureMech_008530 --> ing13_nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing13_nitrogen_gas -.-> CHEBI_17997
    ing14_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_008530 --> ing14_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_2H2O -.-> CHEBI_75213
    ing15_H3BO3["H3BO3"]
    CultureMech_008530 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_008530 --> ing16_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_4H2O -.-> CHEBI_86368
    ing17_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_008530 --> ing17_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing17_CoCl2_6H2O -.-> CHEBI_35696
    ing18_NiCl2_6H2O["NiCl2·6H2O"]
    CultureMech_008530 --> ing18_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_6H2O -.-> CHEBI_34887
    ing19_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_008530 --> ing19_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_2H2O -.-> CHEBI_86318
    ing20_ZnCl2["ZnCl2"]
    CultureMech_008530 --> ing20_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing20_ZnCl2 -.-> CHEBI_49976
    ing21_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
    CultureMech_008530 --> ing21_HCl__25___7_7_M_
    ing22_FeCl2_4H2O["FeCl2·4H2O"]
    CultureMech_008530 --> ing22_FeCl2_4H2O
    CHEBI_86249["CHEBI:86249"]
    ing22_FeCl2_4H2O -.-> CHEBI_86249

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 Vitamin B12 (0.002% (w/v)) 1 G_PER_L Role: Vitamin; Properties: Defined component, Organic compound, Solution
2 Distilled water
H2O 18.015 g/mol
991.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 990.0]
3 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 Yeast extract 0.4 G_PER_L Role: Nutrient source; Properties: Undefined component
5 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
8 NH4Cl
Cl.H4N 53.492 g/mol
0.34 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
9 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
10 Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
0.48 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
11 Sodium acetate
C2H3O2.Na 82.034 g/mol
2 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Organic compound, Defined component, Simple component
12 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
2 G_PER_L CHEBI:50144
sodium pyruvate
 Role: Carbon source; Properties: Organic compound, Defined component, Simple component
13 Sodium succinate
C4H4O4.2Na 162.052 g/mol
2 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
14 nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
15 Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
40 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16 H3BO3
H3BO3 61.833 g/mol
60 G_PER_L CHEBI:33118
boric acid
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17 MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
100 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18 CoCl2·6H2O
2Cl.Co 129.839 g/mol
200 G_PER_L CHEBI:35696
cobalt dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
19 NiCl2·6H2O
Cl2Ni 129.599 g/mol
20 G_PER_L CHEBI:34887
nickel dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
20 CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
20 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21 ZnCl2
2Cl.Zn 136.296 g/mol
70 G_PER_L CHEBI:49976
zinc dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22 HCl (25%; 7.7 M) 10 G_PER_L Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
23 FeCl2·4H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component

Solutions

Ferric citrate solution* (0.1%)

Dose: 5 G_PER_L

Role: Mineral source; Properties: Defined component, Organic compound, Solution

NaHCO3 solution (10% w/v)

Dose: 15 G_PER_L

Role: Buffer; Properties: Solution, Inorganic compound, Defined component

Trace element solution SL7**

Dose: 1 G_PER_L

See mediadive_2739_Trace_element_solution_SL7.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:58.726586Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1950
2026-01-28T19:48:17.427383Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: nitrogen gas
2026-03-09T08:04:28.864807+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:08:57.676225Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:57.800997Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008530
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:02.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.424317+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Medium for Strain JA746" to "medium_for_strain_ja746" for consistent matching
2026-05-16T09:04:20.475663+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:29.597975+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:21.413211+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:06.247175+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:43.614605+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 4e51911a07ba0ba6c87f321667797f74cb94fe226717d143db6a960d688483c6