medium_for_strain_ja747

CultureMech:008533 bacterial COMPLEX LIQUID
Original name: Medium for Strain JA747

Overview

External ontology
TOGO:M1953 — Medium for Strain JA747
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1953 Original source: NBRC - NBRC_M1230 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1230

Composition

flowchart LR
    CultureMech_008533["medium_for_strain_ja747"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008533 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008533 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_008533 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008533 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_008533 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_008533 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Dextrose["Dextrose"]
    CultureMech_008533 --> ing6_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing6_Dextrose -.-> CHEBI_17634
    ing7_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_008533 --> ing7_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_6H2O -.-> CHEBI_86345
    ing8_KCl["KCl"]
    CultureMech_008533 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_Na2SO4["Na2SO4"]
    CultureMech_008533 --> ing9_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing9_Na2SO4 -.-> CHEBI_32149
    ing10_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_008533 --> ing10_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing10_Sodium_pyruvate -.-> CHEBI_50144
    ing11_HEPES["HEPES"]
    CultureMech_008533 --> ing11_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing11_HEPES -.-> CHEBI_46756
    ing12_Beef_extract["Beef extract"]
    CultureMech_008533 --> ing12_Beef_extract
    ing13_Casein_hydrolysate["Casein hydrolysate"]
    CultureMech_008533 --> ing13_Casein_hydrolysate
    ing14_Peptone["Peptone"]
    CultureMech_008533 --> ing14_Peptone

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 Yeast extract 3 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
6 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
7 Dextrose
C6H12O6 180.156 g/mol
0.05 G_PER_L CHEBI:17634
D-glucose
Role: Carbon source; Properties: Organic compound, Simple component, Defined component
8 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.62 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
9 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 Na2SO4
2Na.O4S 142.043 g/mol
2.84 G_PER_L CHEBI:32149
sodium sulfate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
11 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
3 G_PER_L CHEBI:50144
sodium pyruvate
Role: Carbon source; Properties: Defined component, Simple component, Organic compound
12 HEPES 2.38 G_PER_L CHEBI:46756
HEPES
Role: Buffer; Properties: Defined component, Simple component, Organic compound
13 Beef extract 3 G_PER_L Properties: Undefined component
14 Casein hydrolysate 0.05 G_PER_L Properties: Undefined component
15 Peptone 3 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:58.735458Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1953
2026-01-28T19:48:16.554393Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:57.836848Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008533
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.461434+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Medium for Strain JA747" to "medium_for_strain_ja747" for consistent matching
2026-06-05T07:41:29.609782+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.253534+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:43.628225+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 97a9be1a8415b2cd7ed2fd03da5e551e66dc920b6045cc6488a8bc314e0ec791