Overview
- External ontology
- TOGO:M1955 — Modified 288 medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1955 Original source: NBRC - NBRC_M1233 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1233
Composition
flowchart LR
CultureMech_008535["modified_288_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008535 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008535 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NH4Cl["NH4Cl"]
CultureMech_008535 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_K2HPO4["K2HPO4"]
CultureMech_008535 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Sodium_lactate["Sodium lactate"]
CultureMech_008535 --> ing4_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing4_Sodium_lactate -.-> CHEBI_75228
ing5_Ferric_citrate["Ferric citrate"]
CultureMech_008535 --> ing5_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing5_Ferric_citrate -.-> CHEBI_144421
ing6_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008535 --> ing6_Bacto_Yeast_Extract__Difco_
ing7_Sodium_thioglycolate["Sodium thioglycolate"]
CultureMech_008535 --> ing7_Sodium_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing7_Sodium_thioglycolate -.-> CHEBI_86481
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
2.6 G_PER_L |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 6 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
0.006 G_PER_L |
CHEBI:144421
iron(III) citrate
|
Role: Mineral source; Properties: Defined component, Organic compound, Simple component |
| 7 | Bacto Yeast Extract (Difco) | 0.1 G_PER_L | Properties: Undefined component | |
| 8 |
Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.5 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Properties: Defined component, Simple component, Organic compound |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_288_medium (CultureMech:008535)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.738424Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1955 |
| 2026-01-28T19:48:40.737133Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:57.847986Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008535 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.474045+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified 288 medium" to "modified_288_medium" for consistent matching |
| 2026-06-05T07:41:36.027499+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:25.566663+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.459821+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.800615+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c89a892d6585a577903df934ce74c27d98101bd32358af06550c67706350c125 |