Overview
- External ontology
- TOGO:M1994 — Modified 289 medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1994 Original source: NBRC - NBRC_M1280 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1280
Composition
flowchart LR
CultureMech_008578["modified_289_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008578 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_008578 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_008578 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Sodium_succinate["Sodium succinate"]
CultureMech_008578 --> ing3_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing3_Sodium_succinate -.-> CHEBI_63675
ing4_Sodium_lactate["Sodium lactate"]
CultureMech_008578 --> ing4_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing4_Sodium_lactate -.-> CHEBI_75228
ing5_Sodium_tartrate["Sodium tartrate"]
CultureMech_008578 --> ing5_Sodium_tartrate
CHEBI_63017["CHEBI:63017"]
ing5_Sodium_tartrate -.-> CHEBI_63017
ing6_Sodium_thioglycolate["Sodium thioglycolate"]
CultureMech_008578 --> ing6_Sodium_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing6_Sodium_thioglycolate -.-> CHEBI_86481
ing7_NaOH["NaOH"]
CultureMech_008578 --> ing7_NaOH
CHEBI_32145["CHEBI:32145"]
ing7_NaOH -.-> CHEBI_32145
ing8_Biotin["Biotin"]
CultureMech_008578 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008578 --> ing9_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing9_p_Aminobenzoic_acid -.-> CHEBI_30753
ing10_Thiamin_HCl["Thiamin-HCl"]
CultureMech_008578 --> ing10_Thiamin_HCl
CHEBI_49105["CHEBI:49105"]
ing10_Thiamin_HCl -.-> CHEBI_49105
ing11_Ca_pantothenate["Ca-pantothenate"]
CultureMech_008578 --> ing11_Ca_pantothenate
CHEBI_29032["CHEBI:29032"]
ing11_Ca_pantothenate -.-> CHEBI_29032
ing12_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_008578 --> ing12_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing12_Pyridoxine_HCl -.-> CHEBI_30961
ing13_Folic_acid["Folic acid"]
CultureMech_008578 --> ing13_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing13_Folic_acid -.-> CHEBI_27470
ing14_Cyanocobalamin["Cyanocobalamin"]
CultureMech_008578 --> ing14_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing14_Cyanocobalamin -.-> CHEBI_17439
ing15_Riboflavin["Riboflavin"]
CultureMech_008578 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Niacin["Niacin"]
CultureMech_008578 --> ing16_Niacin
CHEBI_15940["CHEBI:15940"]
ing16_Niacin -.-> CHEBI_15940
ing17_Choline_chloride["Choline chloride"]
CultureMech_008578 --> ing17_Choline_chloride
CHEBI_133341["CHEBI:133341"]
ing17_Choline_chloride -.-> CHEBI_133341
ing18_myo_Inositol["myo-Inositol"]
CultureMech_008578 --> ing18_myo_Inositol
CHEBI_17268["CHEBI:17268"]
ing18_myo_Inositol -.-> CHEBI_17268
ing19_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_008578 --> ing19_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing19_FeCl3_6H2O -.-> CHEBI_86254
ing20_Quinic_acid["Quinic acid"]
CultureMech_008578 --> ing20_Quinic_acid
CHEBI_26493["CHEBI:26493"]
ing20_Quinic_acid -.-> CHEBI_26493
ing21_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008578 --> ing21_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing21_MgSO4_7H2O -.-> CHEBI_31795
ing22_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008578 --> ing22_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing22_CaCl2_2H2O -.-> CHEBI_86158
ing23_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008578 --> ing23_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_2H2O -.-> CHEBI_75213
ing24_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008578 --> ing24_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing24_FeSO4_7H2O -.-> CHEBI_75836
ing25_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_008578 --> ing25_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing25_Nitrilotriacetic_acid -.-> CHEBI_44557
ing26_KOH["KOH"]
CultureMech_008578 --> ing26_KOH
CHEBI_32035["CHEBI:32035"]
ing26_KOH -.-> CHEBI_32035
ing27_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008578 --> ing27_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing27_CoCl2_6H2O -.-> CHEBI_35696
ing28_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008578 --> ing28_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing28_ZnSO4_7H2O -.-> CHEBI_32312
ing29_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008578 --> ing29_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing29_CuSO4_5H2O -.-> CHEBI_31440
CultureMech_008578 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
5.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 1.0, 1.0, 1.0, 1.0, 1.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.12 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.37 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 5 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.05 G_PER_L |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 |
Sodium tartrate
C4H4O6.2Na 194.05 g/mol
|
0.37 G_PER_L |
CHEBI:63017
sodium L-tartrate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 7 |
Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.05 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Properties: Defined component, Organic compound, Simple component |
| 8 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 10 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
20 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 11 |
Thiamin-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
20 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 12 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
40 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
40 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
2 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
40 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
Niacin
C6H5NO2 123.111 g/mol
|
40 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Choline chloride
C5H14NO.Cl 139.626 g/mol
|
100 G_PER_L |
CHEBI:133341
choline chloride
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 19 |
myo-Inositol
C6H12O6 180.156 g/mol
|
200 G_PER_L |
CHEBI:17268
myo-inositol
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 20 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.45 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 | Quinic acid | 0.19 G_PER_L |
CHEBI:26493
quinic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 22 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
12.7 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
104.0 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 99.0, 5.0] |
| 26 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 27 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 28 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.203 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
10.95 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.392 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
MnSO4·nH2O
Mn.O4S 151.001 g/mol
|
1.54 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
Na2B4O7·10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.177 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
EDTA
C10H16N2O8 292.244 g/mol
|
2.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Simple component, Organic compound |
Solutions
Vitamin mixture*
Dose: 5 G_PER_L
See mediadive_5209_Vitamin_mixture.yaml for composition
Ferric quinate solution**
Dose: 2 G_PER_L
See mediadive_4559_Ferric_quinate_solution.yaml for composition
HMSS solution***
Dose: 1 G_PER_L
Metal 44 solution****
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_289_medium (CultureMech:008578)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.823142Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1994 |
| 2026-01-28T19:48:32.972922Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:54.576659+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:26.110303Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:58.285864Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008578 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.907169+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified 289 medium" to "modified_289_medium" for consistent matching |
| 2026-05-16T09:04:24.929336+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:36.044305+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.660618+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:58:25.583174+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.462801+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.822572+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c195c8feef26452d89b704740a68fa226f66f4e124ddc0653695072d1adb16c7 |