Overview
- External ontology
- TOGO:M203 — Modified Medium 10
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M203 Original source: JCM - JCM_M210 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=210
Composition
flowchart LR
CultureMech_008629["modified_medium_10"]
ing0_Distilled_water["Distilled water"]
CultureMech_008629 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Cellobiose["Cellobiose"]
CultureMech_008629 --> ing1_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing1_Cellobiose -.-> CHEBI_17057
ing2_Glucose["Glucose"]
CultureMech_008629 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Soluble_starch["Soluble starch"]
CultureMech_008629 --> ing3_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing3_Soluble_starch -.-> CHEBI_28017
ing4_Agar["Agar"]
CultureMech_008629 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008629 --> ing5_Yeast_extract__BD_Difco_
ing6_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_008629 --> ing6_Trypticase_peptone__BD_BBL_
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
850 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.5 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 4 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 5 | Agar | 18 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 6 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 7 | Trypticase peptone (BD-BBL) | 2 G_PER_L | Properties: Undefined component |
Solutions
0.1% Resazurin solution
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Solution, Defined component
1.0% Hemin solution
Dose: 1 G_PER_L
Role: Growth factor; Properties: Solution, Organic compound, Defined component
4% Na2SO3 solution
Dose: 100 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
25% L--Ascorbic acid solution
Dose: 2 G_PER_L
Role: Vitamin; Properties: Organic compound, Solution, Defined component
5% L--Cysteine・HCl・H2O solution
Dose: 10 G_PER_L
Properties: Defined component, Organic compound, Solution
VFA solution (see Medium [M124])
Dose: 3.1 G_PER_L
Cross-reference to Medium M124
Salt solution No. 1 (see Medium [M124])
Dose: 37.5 G_PER_L
Cross-reference to Medium M124
Salt solution No. 2 (see Medium [M124])
Dose: 37.5 G_PER_L
Cross-reference to Medium M124
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M203_Modified_Medium_10 (CultureMech:008629)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.605732Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M203 |
| 2026-01-28T19:48:31.851627Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:53.041477+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T06:19:58.855911Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008629 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.478055+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Medium 10" to "modified_medium_10" for consistent matching |
| 2026-05-16T09:03:12.228666+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.377291+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:10.393693+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f2d0685310c86af6ce74628b16e2092f248e24edee1374023a7182a8d5ec6b71 |