Overview
- External ontology
- TOGO:M2041 — M9-Phenanthrene
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2041 Original source: NBRC - NBRC_M1339 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1339
Composition
flowchart LR
CultureMech_008631["m9_phenanthrene"]
ing0_distilled_water["distilled water"]
CultureMech_008631 --> ing0_distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_distilled_water -.-> CHEBI_15377
ing1_agar["agar"]
CultureMech_008631 --> ing1_agar
CHEBI_2509["CHEBI:2509"]
ing1_agar -.-> CHEBI_2509
ing2_NaCl["NaCl"]
CultureMech_008631 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KH2PO4["KH2PO4"]
CultureMech_008631 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_008631 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Na2HPO4["Na2HPO4"]
CultureMech_008631 --> ing5_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing5_Na2HPO4 -.-> CHEBI_34683
ing6_dimethyl_sulfoxide__DMSO_["dimethyl sulfoxide (DMSO)"]
CultureMech_008631 --> ing6_dimethyl_sulfoxide__DMSO_
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
distilled water
H2O 18.015 g/mol
|
901.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Defined component, Inorganic compound [Merged 2 duplicates: 900.0, 1.0] |
| 2 | agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
30 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
10 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
60 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 7 | dimethyl sulfoxide (DMSO) | variable VARIABLE | Role: Solvating media; Properties: Defined component, Organic compound, Simple component |
Solutions
CaCl2 solution (0.1 M)***
Dose: 1 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Inorganic compound
MgSO4 solution (1 M)***
Dose: 2.1 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound
Agar solution*
Dose: 900 G_PER_L
10xM9 solution**
Dose: 100 G_PER_L
Phenanthrene solution (250 mM) in dimethyl sulfoxide (DMSO)****
Dose: 10 G_PER_L
Phenanthrene solution
Dose: variable VARIABLE
Role: Carbon source
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- m9_phenanthrene (CultureMech:008631)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.940796Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2041 |
| 2026-01-28T19:48:22.219404Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Phenanthrene solution; Added default concentration for ingredient: dimethyl sulfoxide (DMSO) |
| 2026-03-09T08:04:37.094194+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:06.616427Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:58.870104Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008631 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.492986+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M9-Phenanthrene" to "m9_phenanthrene" for consistent matching |
| 2026-05-16T09:03:39.656246+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:12.082682+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.723721+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:41.242142+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 23e3584c96689e72d8200e5ed225315790d8bafaa346bfa4ace13d146ebc651e |