medium_for_strain_ttn

CultureMech:008633 bacterial COMPLEX LIQUID
Original name: Medium for Strain TTN

Overview

External ontology
TOGO:M2043 — Medium for Strain TTN
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2043 Original source: NBRC - NBRC_M1341 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1341

Composition

flowchart LR
    CultureMech_008633["medium_for_strain_ttn"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008633 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008633 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008633 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008633 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008633 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_FeCl3_6H2O["FeCl3·6H2O"]
    CultureMech_008633 --> ing5_FeCl3_6H2O
    CHEBI_86254["CHEBI:86254"]
    ing5_FeCl3_6H2O -.-> CHEBI_86254
    ing6_Na2HPO4_12H2O["Na2HPO4·12H2O"]
    CultureMech_008633 --> ing6_Na2HPO4_12H2O
    CHEBI_34683["CHEBI:34683"]
    ing6_Na2HPO4_12H2O -.-> CHEBI_34683
    ing7_MnSO4_5H2O["MnSO4·5H2O"]
    CultureMech_008633 --> ing7_MnSO4_5H2O
    CHEBI_131524["CHEBI:131524"]
    ing7_MnSO4_5H2O -.-> CHEBI_131524
    ing8_Na2S2O3["Na2S2O3"]
    CultureMech_008633 --> ing8_Na2S2O3
    CHEBI_32150["CHEBI:32150"]
    ing8_Na2S2O3 -.-> CHEBI_32150
    ing9_CO2["CO2"]
    CultureMech_008633 --> ing9_CO2
    CHEBI_16526["CHEBI:16526"]
    ing9_CO2 -.-> CHEBI_16526

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.04 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 KH2PO4
H2O4P.K 136.084 g/mol
1.8 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.1 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
6 FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
0.03 G_PER_L CHEBI:86254
iron trichloride hexahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7 Na2HPO4·12H2O
HO4P.2Na 141.958 g/mol
3 G_PER_L CHEBI:34683
disodium hydrogenphosphate
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
8 MnSO4·5H2O
5H2O.Mn.O4S 241.076 g/mol
0.03 G_PER_L CHEBI:131524
manganese(II) sulfate pentahydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
9 Na2S2O3
5H2O.2Na.O3S2 248.186 g/mol
10 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
Properties: Defined component, Inorganic compound, Simple component
10 CO2
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: Gas

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:58.943324Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2043
2026-01-28T19:48:21.167225Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: CO2
2026-03-13T06:19:58.881956Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008633
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.504955+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Medium for Strain TTN" to "medium_for_strain_ttn" for consistent matching
2026-05-16T09:04:20.788427+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:29.916306+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:21.651447+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:06.373113+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:43.948527+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ebb8d448981be7c1c198cd797ed07cfed21134f4d5c7c43ce1c824ad5269737c