medium_for_strain_ha5 (CultureMech:008635)

Overview

External ontology

TOGO:M2045 — Medium for Strain HA5

Applications

Microbial cultivation

High-metal flag

True

Notes

Source: https://togomedium.org/medium/M2045 Original source: NBRC - NBRC_M1343 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1343

Composition

flowchart LR
    CultureMech_008635["medium_for_strain_ha5"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008635 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008635 --> ing1_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_2H2O -.-> CHEBI_86158
    ing2_KH2PO4["KH2PO4"]
    CultureMech_008635 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_008635 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_008635 --> ing4_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_6H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_008635 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_008635 --> ing6_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing6_Na2MoO4_2H2O -.-> CHEBI_75213
    ing7_H3BO3["H3BO3"]
    CultureMech_008635 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118
    ing8_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_008635 --> ing8_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_7H2O -.-> CHEBI_75836
    ing9_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_008635 --> ing9_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_4H2O -.-> CHEBI_86368
    ing10_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_008635 --> ing10_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing10_CoCl2_6H2O -.-> CHEBI_35696
    ing11_NiCl2_6H2O["NiCl2·6H2O"]
    CultureMech_008635 --> ing11_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing11_NiCl2_6H2O -.-> CHEBI_34887
    ing12_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_008635 --> ing12_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_7H2O -.-> CHEBI_32312
    ing13_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_008635 --> ing13_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_2H2O -.-> CHEBI_86318
    ing14_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
    CultureMech_008635 --> ing14_HCl__25___7_7_M_
    ing15_Na2SeO3_5H2O["Na2SeO3·5H2O"]
    CultureMech_008635 --> ing15_Na2SeO3_5H2O
    CHEBI_131361["CHEBI:131361"]
    ing15_Na2SeO3_5H2O -.-> CHEBI_131361
    ing16_Na2WO4_2H2O["Na2WO4·2H2O"]
    CultureMech_008635 --> ing16_Na2WO4_2H2O
    CHEBI_63939["CHEBI:63939"]
    ing16_Na2WO4_2H2O -.-> CHEBI_63939
    ing17_NaOH["NaOH"]
    CultureMech_008635 --> ing17_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing17_NaOH -.-> CHEBI_32145
    ing18_Biotin["Biotin"]
    CultureMech_008635 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_008635 --> ing19_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing20_Thiamine_HCl["Thiamine-HCl"]
    CultureMech_008635 --> ing20_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing20_Thiamine_HCl -.-> CHEBI_49105
    ing21_Ca_pantothenate["Ca-pantothenate"]
    CultureMech_008635 --> ing21_Ca_pantothenate
    CHEBI_29032["CHEBI:29032"]
    ing21_Ca_pantothenate -.-> CHEBI_29032
    ing22_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_008635 --> ing22_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_HCl -.-> CHEBI_30961
    ing23_Folic_acid["Folic acid"]
    CultureMech_008635 --> ing23_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing23_Folic_acid -.-> CHEBI_27470
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_008635 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843
    ing25_Riboflavin["Riboflavin"]
    CultureMech_008635 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_008635 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_Lipoic_acid["Lipoic acid"]
    CultureMech_008635 --> ing27_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing27_Lipoic_acid -.-> CHEBI_16494
    ing28_NaHCO3["NaHCO3"]
    CultureMech_008635 --> ing28_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing28_NaHCO3 -.-> CHEBI_32139
    ing29_CO2_gas["CO2 gas"]
    CultureMech_008635 --> ing29_CO2_gas
    CultureMech_008635 --> more["...2 more"]

Ingredients (32)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	2139.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 6 duplicates: 950.0, 987.0, 1.0, 1.0, 100.0, 100.0]
2	CaCl2·2H2O Ca.2Cl.2H2O 147.014 g/mol	0.1 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3	KH2PO4 H2O4P.K 136.084 g/mol	0.1 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
4	NH4Cl Cl.H4N 53.492 g/mol	0.1 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
5	MgCl2·6H2O 2Cl.6H2O.Mg 203.301 g/mol	0.2 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6	KCl Cl.K 74.551 g/mol	0.1 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7	Na2MoO4·2H2O 2H2O.MoO4.2Na 241.946 g/mol	36 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8	H3BO3 H3BO3 61.833 g/mol	30 G_PER_L	CHEBI:33118 boric acid	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9	FeSO4·7H2O Fe.7H2O.O4S 278.013 g/mol	2100 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10	MnCl2·4H2O Cl2Mn.4H2O 197.904 g/mol	100 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11	CoCl2·6H2O 2Cl.Co 129.839 g/mol	190 G_PER_L	CHEBI:35696 cobalt dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12	NiCl2·6H2O Cl2Ni 129.599 g/mol	24 G_PER_L	CHEBI:34887 nickel dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13	ZnSO4·7H2O 7H2O.O4S.Zn 287.558 g/mol	144 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14	CuCl2·2H2O Cl2Cu.2H2O 170.482 g/mol	2 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15	HCl (25%; 7.7 M)	12.5 G_PER_L		Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
16	Na2SeO3·5H2O 5H2O.2Na.O3Se 263.012 g/mol	6 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17	Na2WO4·2H2O 2H2O.2Na.O4W 329.846 g/mol	8 G_PER_L	CHEBI:63939 sodium tungstate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18	NaOH HO.Na 39.997 g/mol	0.4 G_PER_L	CHEBI:32145 sodium hydroxide	Properties: Defined component, Simple component, Inorganic compound
19	Biotin C10H16N2O3S 244.316 g/mol	2 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
20	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	5 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
21	Thiamine-HCl C12H17N4OS.Cl.HCl 337.276 g/mol	5 G_PER_L	CHEBI:49105 thiamine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
22	Ca-pantothenate C9H16NO5 218.229 g/mol	5 G_PER_L	CHEBI:29032 (R)-pantothenate	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
23	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	10 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
24	Folic acid C19H19N7O6 441.404 g/mol	2 G_PER_L	CHEBI:27470 folic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
25	Vitamin B12	0.1 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
26	Riboflavin C17H20N4O6 376.369 g/mol	5 G_PER_L	CHEBI:17015 riboflavin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
27	Nicotinic acid C6H5NO2 123.111 g/mol	5 G_PER_L	CHEBI:15940 nicotinic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
28	Lipoic acid C8H14O2S2 206.332 g/mol	5 G_PER_L	CHEBI:16494 lipoic acid	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
29	NaHCO3 CHO3.Na 84.006 g/mol	8.4 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
30	CO2 gas	variable VARIABLE		Properties: ガス
31	Na2S2O3·5H2O 5H2O.2Na.O3S2 248.186 g/mol	24.8 G_PER_L	CHEBI:32150 sodium thiosulfate pentahydrate	Properties: Defined component, Inorganic compound, Simple component
32	N2 gas	variable VARIABLE		Properties: ガス

Solutions

Trace element mixture*

Dose: 1 G_PER_L

Selenite-tungstate solution**

Dose: 1 G_PER_L

See mediadive_5543_Selenite-tungstate_solution.yaml for composition

Vitamin solution***

Dose: 1 G_PER_L

See mediadive_6241_Vitamin_solution.yaml for composition

Bicarbonate solution****

Dose: 15 G_PER_L

See mediadive_29_Bicarbonate_solution.yaml for composition

Thiosulfate solution*****

Dose: 20 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

medium_for_strain_ha5 (CultureMech:008635)

Timestamp	Curator	Action	Notes
2026-01-27T06:35:58.950067Z	togo-import	Imported from TOGO Medium	Source: NBRC, ID: M2045
2026-01-28T19:48:33.944931Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: CO2 gas; Added default concentration for ingredient: N2 gas
2026-03-09T08:04:56.331831+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 5 solution(s) from ingredients
2026-03-13T03:09:27.890483Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 5 duplicate ingredient(s)
2026-03-13T06:19:58.920198Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008635
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:37.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.542237+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Medium for Strain HA5" to "medium_for_strain_ha5" for consistent matching
2026-05-16T09:04:20.330932+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:41:29.457883+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:47.129238+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24)	Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:47:06.188610+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:43.446825+00:00	merge_recipes.py	MERGED_RECIPES	merged 1 source recipe(s) on fingerprint b8a00b9ba0fc53efbb2b20d2e5edbc75bc912f04d463d02cd6f771bd5094e3d6