Overview
- External ontology
- TOGO:M2059 — Medium for Strain M6.X2
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2059 Original source: NBRC - NBRC_M1361 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1361
Composition
flowchart LR
CultureMech_008649["medium_for_strain_m6_x2"]
ing0_Distilled_water["Distilled water"]
CultureMech_008649 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008649 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008649 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008649 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_K2HPO4["K2HPO4"]
CultureMech_008649 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Resazurin["Resazurin"]
CultureMech_008649 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Tween_80["Tween 80"]
CultureMech_008649 --> ing6_Tween_80
CHEBI_53426["CHEBI:53426"]
ing6_Tween_80 -.-> CHEBI_53426
ing7_MnSO4_H2O["MnSO4·H2O"]
CultureMech_008649 --> ing7_MnSO4_H2O
CHEBI_86364["CHEBI:86364"]
ing7_MnSO4_H2O -.-> CHEBI_86364
ing8_Glucose["Glucose"]
CultureMech_008649 --> ing8_Glucose
CHEBI_17234["CHEBI:17234"]
ing8_Glucose -.-> CHEBI_17234
ing9_Inulin["Inulin"]
CultureMech_008649 --> ing9_Inulin
CHEBI_15443["CHEBI:15443"]
ing9_Inulin -.-> CHEBI_15443
ing10_Meat_extract["Meat extract"]
CultureMech_008649 --> ing10_Meat_extract
ing11_Bacto_Soytone["Bacto Soytone"]
CultureMech_008649 --> ing11_Bacto_Soytone
ing12_Cysteine_HCl_H2O["Cysteine-HCl·H2O"]
CultureMech_008649 --> ing12_Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing12_Cysteine_HCl_H2O -.-> CHEBI_91248
ing13_Casein_peptone__tryptic_digest["Casein peptone, tryptic digest"]
CultureMech_008649 --> ing13_Casein_peptone__tryptic_digest
ing14_NaOH["NaOH"]
CultureMech_008649 --> ing14_NaOH
CHEBI_32145["CHEBI:32145"]
ing14_NaOH -.-> CHEBI_32145
ing15_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008649 --> ing15_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing15_Carbon_dioxide_gas -.-> CHEBI_16526
ing16_Nitrogen_gas["Nitrogen gas"]
CultureMech_008649 --> ing16_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing16_Nitrogen_gas -.-> CHEBI_17997
ing17_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008649 --> ing17_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing17_CaCl2_2H2O -.-> CHEBI_86158
ing18_KH2PO4["KH2PO4"]
CultureMech_008649 --> ing18_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing18_KH2PO4 -.-> CHEBI_63036
ing19_NaHCO3["NaHCO3"]
CultureMech_008649 --> ing19_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing19_NaHCO3 -.-> CHEBI_32139
ing20_Biotin["Biotin"]
CultureMech_008649 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008649 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_008649 --> ing22_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_HCl -.-> CHEBI_30961
ing23_Folic_acid["Folic acid"]
CultureMech_008649 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_008649 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
ing25_Riboflavin["Riboflavin"]
CultureMech_008649 --> ing25_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing25_Riboflavin -.-> CHEBI_17015
ing26_Nicotinic_acid["Nicotinic acid"]
CultureMech_008649 --> ing26_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing26_Nicotinic_acid -.-> CHEBI_15940
ing27_Thiamine_HCl_2H2O["Thiamine-HCl·2H2O"]
CultureMech_008649 --> ing27_Thiamine_HCl_2H2O
CHEBI_132751["CHEBI:132751"]
ing27_Thiamine_HCl_2H2O -.-> CHEBI_132751
ing28_Lipoic_acid["Lipoic acid"]
CultureMech_008649 --> ing28_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28_Lipoic_acid -.-> CHEBI_16494
ing29_D_Ca_pantothenate["D-Ca-pantothenate"]
CultureMech_008649 --> ing29_D_Ca_pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_D_Ca_pantothenate -.-> CHEBI_31345
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
952.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 950.0, 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.2, 0.5] |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
7.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 5.0, 2.0] |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3.0 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.0, 1.0] |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Organic compound, Defined component, Simple component |
| 8 |
MnSO4·H2O
H2O.Mn.O4S 169.016 g/mol
|
0.05 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 10 |
Inulin
(C12H20O11)n
|
1 G_PER_L |
CHEBI:15443
inulin
|
Role: Carbon source |
| 11 | Meat extract | 5 G_PER_L | Properties: Undefined component | |
| 12 | Bacto Soytone | 5 G_PER_L | Properties: Undefined component | |
| 13 |
Cysteine-HCl·H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component |
| 14 | Casein peptone, tryptic digest | 10 G_PER_L | Properties: Undefined component | |
| 15 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 16 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 17 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 18 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Thiamine-HCl·2H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
5 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 30 |
D-Ca-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Defined component |
Solutions
Salt solution*
Dose: 40 G_PER_L
See mediadive_6218_Salt_solution.yaml for composition
Vitamin solution**
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_for_strain_m6_x2 (CultureMech:008649)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:58.979062Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2059 |
| 2026-01-28T19:48:18.675976Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:30.969430+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:00.012640Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:59.051882Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008649 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.675318+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for Strain M6.X2" to "medium_for_strain_m6_x2" for consistent matching |
| 2026-05-16T09:04:20.554868+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:29.682690+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:47.229257+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:58:21.484842+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:06.276925+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:43.711189+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8690984db364c02bb782c8d2b23083b19a89185abcc59801b82cb8a0dadcb5e7 |