Overview
- External ontology
- TOGO:M2071 — Medium for Acidithiobacillus ferrooxidans
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2071 Original source: NBRC - NBRC_M1379 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1379
Composition
flowchart LR
CultureMech_008660["medium_for_acidithiobacillus_ferrooxidans"]
ing0_Distilled_water["Distilled water"]
CultureMech_008660 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_K2HPO4["K2HPO4"]
CultureMech_008660 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_KCl["KCl"]
CultureMech_008660 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_008660 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_H2SO4["H2SO4"]
CultureMech_008660 --> ing4_H2SO4
CHEBI_26836["CHEBI:26836"]
ing4_H2SO4 -.-> CHEBI_26836
ing5_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008660 --> ing5_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_7H2O -.-> CHEBI_31795
ing6_Ca_NO3_2_4H2O["Ca(NO3)2·4H2O"]
CultureMech_008660 --> ing6_Ca_NO3_2_4H2O
CHEBI_86159["CHEBI:86159"]
ing6_Ca_NO3_2_4H2O -.-> CHEBI_86159
ing7_0_25_N_H2SO4["0.25 N H2SO4"]
CultureMech_008660 --> ing7_0_25_N_H2SO4
ing8_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008660 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
891.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 890.0, 1.0] |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component |
| 6 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
50 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 7 |
Ca(NO3)2·4H2O
Ca.4H2O.2NO3 236.146 g/mol
|
1.44 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 | 0.25 N H2SO4 | 1 G_PER_L | Role: Mineral source; Properties: Defined component, Inorganic compound, Solution | |
| 9 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
400 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
Solutions
Solution I*
Dose: 10 G_PER_L
See mediadive_5335_Solution_I.yaml for composition
Solution II**
Dose: 100 G_PER_L
See mediadive_5336_Solution_II.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_for_acidithiobacillus_ferrooxidans (CultureMech:008660)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.994543Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2071 |
| 2026-01-28T19:48:15.557100Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: H2SO4 |
| 2026-03-09T08:04:25.708906+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:54.231590Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:59.130851Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008660 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.761156+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for Acidithiobacillus ferrooxidans" to "medium_for_acidithiobacillus_ferrooxidans" for consistent matching |
| 2026-05-16T09:04:19.810804+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:28.801431+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:20.840607+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:05.956002+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.961962+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2f5f6c6e227add01e69720a5b358af17962c933a1b75a8040b778c3ce609e0b6 |