Overview
- External ontology
- TOGO:M2073 — PPM
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2073 Original source: NBRC - NBRC_M1381 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1381
Composition
flowchart LR
CultureMech_008662["ppm"]
ing0_Distilled_water["Distilled water"]
CultureMech_008662 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008662 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_MES["MES"]
CultureMech_008662 --> ing2_MES
CHEBI_39010["CHEBI:39010"]
ing2_MES -.-> CHEBI_39010
ing3_NH4H2PO4["NH4H2PO4"]
CultureMech_008662 --> ing3_NH4H2PO4
CHEBI_62982["CHEBI:62982"]
ing3_NH4H2PO4 -.-> CHEBI_62982
ing4_Gelrite__if_needed_["Gelrite (if needed)"]
CultureMech_008662 --> ing4_Gelrite__if_needed_
ing5_Cycloheximide["Cycloheximide"]
CultureMech_008662 --> ing5_Cycloheximide
CHEBI_27641["CHEBI:27641"]
ing5_Cycloheximide -.-> CHEBI_27641
ing6_Pectin["Pectin"]
CultureMech_008662 --> ing6_Pectin
CHEBI_17309["CHEBI:17309"]
ing6_Pectin -.-> CHEBI_17309
ing7_Xylan_from_Beechwood["Xylan from Beechwood"]
CultureMech_008662 --> ing7_Xylan_from_Beechwood
ing8_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008662 --> ing8_Bacto_Yeast_Extract__Difco_
ing9_Bacto_Casamino_Acids__Difco_["Bacto Casamino Acids (Difco)"]
CultureMech_008662 --> ing9_Bacto_Casamino_Acids__Difco_
ing10_HCl__25__["HCl (25%)"]
CultureMech_008662 --> ing10_HCl__25__
ing11_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008662 --> ing11_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_2H2O -.-> CHEBI_75213
ing12_B_OH_3["B(OH)3"]
CultureMech_008662 --> ing12_B_OH_3
CHEBI_33118["CHEBI:33118"]
ing12_B_OH_3 -.-> CHEBI_33118
ing13_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008662 --> ing13_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_4H2O -.-> CHEBI_86368
ing14_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008662 --> ing14_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing14_CoCl2_6H2O -.-> CHEBI_35696
ing15_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008662 --> ing15_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_6H2O -.-> CHEBI_34887
ing16_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008662 --> ing16_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_2H2O -.-> CHEBI_86318
ing17_ZnCl2["ZnCl2"]
CultureMech_008662 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_FeCl2_4H2O["FeCl2·4H2O"]
CultureMech_008662 --> ing18_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing18_FeCl2_4H2O -.-> CHEBI_86249
ing19_Na2WO4["Na2WO4"]
CultureMech_008662 --> ing19_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing19_Na2WO4 -.-> CHEBI_63940
ing20_Na2SeO3["Na2SeO3"]
CultureMech_008662 --> ing20_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing20_Na2SeO3 -.-> CHEBI_48843
ing21_NaOH["NaOH"]
CultureMech_008662 --> ing21_NaOH
CHEBI_32145["CHEBI:32145"]
ing21_NaOH -.-> CHEBI_32145
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | MES | 2 G_PER_L |
CHEBI:39010
MES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 4 |
NH4H2PO4
H2O4P.H4N 115.025 g/mol
|
0.053 G_PER_L |
CHEBI:62982
ammonium dihydrogen phosphate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 | Gelrite (if needed) | 8 G_PER_L | Role: Solidifying component; Properties: Organic compound, Undefined component | |
| 6 |
Cycloheximide
C15H23NO4 281.352 g/mol
|
0.1 G_PER_L |
CHEBI:27641
cycloheximide
|
Role: Antimicrobial agent; Properties: Organic compound, Defined component, Simple component |
| 7 | Pectin | 0.05 G_PER_L |
CHEBI:17309
pectin
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 8 | Xylan from Beechwood | 0.05 G_PER_L | Role: Carbon source; Properties: Undefined component | |
| 9 | Bacto Yeast Extract (Difco) | 0.05 G_PER_L | Properties: Undefined component | |
| 10 | Bacto Casamino Acids (Difco) | 0.05 G_PER_L | Properties: Undefined component | |
| 11 | HCl (25%) | 10 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 12 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
B(OH)3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.19 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
FeCl2·4H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2WO4
2Na.O4W 293.816 g/mol
|
0.004 G_PER_L |
CHEBI:63940
sodium tungstate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.002 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Inorganic compound, Simple component, Defined component |
Solutions
Trace element solution (SL-10)*
Dose: 2 G_PER_L
Se/W solution**
Dose: 2 G_PER_L
See mediadive_4826_Se_W_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ppm (CultureMech:008662)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.999339Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2073 |
| 2026-01-28T19:48:42.558307Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:11.714272+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:43.549695Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:59.156628Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008662 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.787563+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPM" to "ppm" for consistent matching |
| 2026-05-16T09:04:29.404662+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:42.460345+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:29.924740+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:10.376679+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.461707+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7a6a33ba7c6d16b06dfbd91ef3966149fd2d8eb56a32745524fae75f4d920686 |