Overview
- External ontology
- TOGO:M2080 — Leeming & Notman agar (LNA) (Leeming & Notman 1987) in The Yeasts, a Taxonomic Study, 5th edn
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2080 Original source: NBRC - NBRC_M1390 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1390
Composition
flowchart LR
CultureMech_008670["leeming_amp_notman_agar_lna_leeming_amp_notman_1987_in_the_yeasts_a..."]
ing0_Distilled_water["Distilled water"]
CultureMech_008670 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008670 --> ing1_Yeast_extract
ing2_Glycerol["Glycerol"]
CultureMech_008670 --> ing2_Glycerol
CHEBI_17754["CHEBI:17754"]
ing2_Glycerol -.-> CHEBI_17754
ing3_Ox_bile__desiccated["Ox bile, desiccated"]
CultureMech_008670 --> ing3_Ox_bile__desiccated
ing4_Whole_fat_cow_s_milk["Whole-fat cow's milk"]
CultureMech_008670 --> ing4_Whole_fat_cow_s_milk
ing5_Glucose["Glucose"]
CultureMech_008670 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Agar["Agar"]
CultureMech_008670 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Glycerol_monostearate["Glycerol monostearate"]
CultureMech_008670 --> ing7_Glycerol_monostearate
CHEBI_75456["CHEBI:75456"]
ing7_Glycerol_monostearate -.-> CHEBI_75456
ing8_Tween_60["Tween 60"]
CultureMech_008670 --> ing8_Tween_60
CHEBI_53425["CHEBI:53425"]
ing8_Tween_60 -.-> CHEBI_53425
ing9_Bacteriological_peptone["Bacteriological peptone"]
CultureMech_008670 --> ing9_Bacteriological_peptone
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
Glycerol
C3H8O3 92.094 g/mol
|
1 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 4 | Ox bile, desiccated | 8 G_PER_L | Role: Selective agent; Properties: Undefined component | |
| 5 | Whole-fat cow's milk | 10 G_PER_L | Role: Nitrogen source; Properties: Undefined component | |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 | Agar | 12 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 |
Glycerol monostearate
C21H42O4 358.563 g/mol
|
0.5 G_PER_L |
CHEBI:75456
2-stearoylglycerol
|
Properties: Defined component, Organic compound, Simple component |
| 9 |
Tween 60
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H46O6 606.838 g/mol
|
0.5 G_PER_L |
CHEBI:53425
polysorbate 60
|
Properties: Defined component, Organic compound, Simple component |
| 10 | Bacteriological peptone | 10 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- leeming_amp_notman_agar_lna_leeming_amp_notman_1987_in_the_yeasts_a_taxonomic_study_5th_edn (CultureMech:008670)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.018090Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2080 |
| 2026-01-28T19:48:35.252768Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:59.248280Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008670 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.881337+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Leeming & Notman agar (LNA) (Leeming & Notman 1987) in The Yeasts, a Taxonomic Study, 5th edn" to "leeming_amp_notman_agar_lna_leeming_amp_notman_1987_in_the_yeasts_a_taxonomic_study_5th_edn" for consistent matching |
| 2026-06-05T07:41:11.038132+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:40.335807+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:40.146630+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 77a72e2c85c8bc90cc26fad91ff4ed5086e29a443dbaada730c9722cede848e0 |