Overview
- External ontology
- TOGO:M2084 — PVA medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2084 Original source: NBRC - NBRC_M1394 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1394
Composition
flowchart LR
CultureMech_008674["pva_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008674 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008674 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008674 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008674 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KH2PO4["KH2PO4"]
CultureMech_008674 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_008674 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_CaCl2["CaCl2"]
CultureMech_008674 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_NH4NO3["NH4NO3"]
CultureMech_008674 --> ing7_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing7_NH4NO3 -.-> CHEBI_63038
ing8_FeSO4["FeSO4"]
CultureMech_008674 --> ing8_FeSO4
CHEBI_75832["CHEBI:75832"]
ing8_FeSO4 -.-> CHEBI_75832
ing9_Pyroroquinoline_quinone__PQQ_["Pyroroquinoline quinone (PQQ)"]
CultureMech_008674 --> ing9_Pyroroquinoline_quinone__PQQ_
ing10_Agar__if_needed_["Agar (if needed)"]
CultureMech_008674 --> ing10_Agar__if_needed_
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
10 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 8 |
NH4NO3
H4N.NO3 80.043 g/mol
|
1 G_PER_L |
CHEBI:63038
ammonium nitrate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
FeSO4
Fe.O4S 151.908 g/mol
|
0.01 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 10 | Pyroroquinoline quinone (PQQ) | 1 G_PER_L | Role: Growth factor; Properties: Organic compound, Simple component, Defined component | |
| 11 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component |
Solutions
PVA117**
Dose: 5 G_PER_L
Role: Carbon source; Properties: Simple component, Defined component
Hipolypepton*
Dose: 0.05 G_PER_L
Properties: Undefined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pva_medium (CultureMech:008674)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.026234Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2084 |
| 2026-01-28T19:48:29.021956Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:48.118834+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:59.285940Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008674 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.920976+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PVA medium" to "pva_medium" for consistent matching |
| 2026-05-16T09:04:29.672133+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:42.866630+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:30.184993+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:10.524921+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.807318+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 30074de66f1d9646b6d04124212aeaa631a4126f0b910671043781dcb469b073 |