Overview
- External ontology
- TOGO:M2088 — Modified Melin Norkrans (MMN) Media
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2088 Original source: NBRC - NBRC_M1398 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1398
Composition
flowchart LR
CultureMech_008678["modified_melin_norkrans_mmn_media"]
ing0_Distilled_water["Distilled water"]
CultureMech_008678 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Potassium_Phosphate_monobasic_crystal["Potassium Phosphate monobasic crystal"]
CultureMech_008678 --> ing1_Potassium_Phosphate_monobasic_crystal
ing2_CaCl2["CaCl2"]
CultureMech_008678 --> ing2_CaCl2
CHEBI_3312["CHEBI:3312"]
ing2_CaCl2 -.-> CHEBI_3312
ing3_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_008678 --> ing3_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing3_Magnesium_sulfate -.-> CHEBI_32599
ing4_Amonium_phosphate_dibasic["Amonium phosphate-dibasic"]
CultureMech_008678 --> ing4_Amonium_phosphate_dibasic
ing5_Glucose["Glucose"]
CultureMech_008678 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Agar["Agar"]
CultureMech_008678 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Malt_extract["Malt extract"]
CultureMech_008678 --> ing7_Malt_extract
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Potassium Phosphate monobasic crystal | 0.5 G_PER_L | Role: Buffer; Properties: Inorganic compound, Simple component, Defined component | |
| 3 |
CaCl2
Ca.2Cl 110.984 g/mol
|
50 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.15 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 | Amonium phosphate-dibasic | 0.25 G_PER_L | Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component | |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Malt extract | 3 G_PER_L | Properties: Undefined component |
Solutions
FeCl3 (1% aqueous solution)
Dose: 1 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_melin_norkrans_mmn_media (CultureMech:008678)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.032235Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2088 |
| 2026-01-28T19:48:08.183441Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:13.170418+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:59.310137Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008678 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.946954+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Melin Norkrans (MMN) Media" to "modified_melin_norkrans_mmn_media" for consistent matching |
| 2026-05-16T09:04:25.779039+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:37.176436+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:48.890506+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 116d950893dc289ebe1532db905e0c0f4e8a8c14b196ed8af724881a4ded3bc0 |