Overview
- External ontology
- TOGO:M2094 — NHB Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2094 Original source: NBRC - NBRC_M1405 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1405
Composition
flowchart LR
CultureMech_008685["nhb_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008685 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008685 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008685 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008685 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_008685 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_KNO3["KNO3"]
CultureMech_008685 --> ing5_KNO3
CHEBI_63043["CHEBI:63043"]
ing5_KNO3 -.-> CHEBI_63043
ing6_Trisodium_citrate["Trisodium citrate"]
CultureMech_008685 --> ing6_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing6_Trisodium_citrate -.-> CHEBI_53258
ing7_Agar__if_needed_["Agar (if needed)"]
CultureMech_008685 --> ing7_Agar__if_needed_
ing8_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008685 --> ing8_Bacto_Yeast_Extract__Difco_
ing9_Bacto_Casamino_Acid__Difco_["Bacto Casamino Acid (Difco)"]
CultureMech_008685 --> ing9_Bacto_Casamino_Acid__Difco_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
10 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
240 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
KCl
Cl.K 74.551 g/mol
|
5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 8 | Agar (if needed) | 20 G_PER_L | Role: Solidifying component | |
| 9 | Bacto Yeast Extract (Difco) | 1 G_PER_L | Properties: Undefined component | |
| 10 | Bacto Casamino Acid (Difco) | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- nhb_medium (CultureMech:008685)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.046263Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2094 |
| 2026-01-28T19:48:10.257345Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:59.389865Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008685 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.026436+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NHB Medium" to "nhb_medium" for consistent matching |
| 2026-06-05T07:41:39.657470+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:28.126957+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:51.328436+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6766fe1a2e620571325bdcac84c30f399531691b9d596a4467e189912d3c301d |