Overview
- External ontology
- TOGO:M2123 — GYP medium (pH 6) for anaerobe
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2123 Original source: NBRC - NBRC_M1451 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1451
Composition
flowchart LR
CultureMech_008716["gyp_medium_ph_6_for_anaerobe"]
ing0_Distilled_water["Distilled water"]
CultureMech_008716 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Resazurin["Resazurin"]
CultureMech_008716 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Sodium_acetate["Sodium acetate"]
CultureMech_008716 --> ing2_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing2_Sodium_acetate -.-> CHEBI_32954
ing3_Glucose["Glucose"]
CultureMech_008716 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008716 --> ing4_Bacto_Yeast_Extract__Difco_
ing5_Cysteine_HCl_H2O["Cysteine-HCl·H2O"]
CultureMech_008716 --> ing5_Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing5_Cysteine_HCl_H2O -.-> CHEBI_91248
ing6_N2["N2"]
CultureMech_008716 --> ing6_N2
CHEBI_17997["CHEBI:17997"]
ing6_N2 -.-> CHEBI_17997
ing7_Tween_80["Tween 80"]
CultureMech_008716 --> ing7_Tween_80
CHEBI_53426["CHEBI:53426"]
ing7_Tween_80 -.-> CHEBI_53426
ing8_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008716 --> ing8_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_7H2O -.-> CHEBI_31795
ing9_NaCl["NaCl"]
CultureMech_008716 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008716 --> ing10_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_7H2O -.-> CHEBI_75836
ing11_MnSO4_4H2O["MnSO4·4H2O"]
CultureMech_008716 --> ing11_MnSO4_4H2O
CHEBI_86358["CHEBI:86358"]
ing11_MnSO4_4H2O -.-> CHEBI_86358
ing12_HCl["HCl"]
CultureMech_008716 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 3 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
2 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 5 | Bacto Yeast Extract (Difco) | 10 G_PER_L | Properties: Undefined component | |
| 6 |
Cysteine-HCl·H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
3 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Organic compound, Simple component, Defined component |
| 7 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 8 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
50 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Organic compound, Simple component |
| 9 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
40 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 11 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 12 |
MnSO4·4H2O
4H2O.Mn.O4S 223.061 g/mol
|
2 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 13 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
Solutions
Hipolypepton*
Dose: 5 G_PER_L
Properties: Undefined component
Tween 80 solution**
Dose: 10 G_PER_L
Salts solution***
Dose: 5 G_PER_L
See mediadive_4429_Salts_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- gyp_medium_ph_6_for_anaerobe (CultureMech:008716)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.121687Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2123 |
| 2026-01-28T19:48:32.744076Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: HCl |
| 2026-03-09T08:04:54.268960+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:25.788377Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:59.757803Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008716 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.397804+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GYP medium (pH 6) for anaerobe" to "gyp_medium_ph_6_for_anaerobe" for consistent matching |
| 2026-05-16T09:03:36.126327+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:06.918876+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:47.728164+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:36.710505+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 356f60ed5262d45ae38d1f3878c9b7ab37ea454d9dda7a17aff6a63c8d4c7b21 |