Overview
- External ontology
- TOGO:M2131 — modified-VY/2-Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2131 Original source: NBRC - NBRC_M1463 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1463
Composition
flowchart LR
CultureMech_008725["modified_vy_2_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_008725 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008725 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008725 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008725 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008725 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_H3BO3["H3BO3"]
CultureMech_008725 --> ing5_H3BO3
CHEBI_33118["CHEBI:33118"]
ing5_H3BO3 -.-> CHEBI_33118
ing6_NaHCO3["NaHCO3"]
CultureMech_008725 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_cyanocobalamin["cyanocobalamin"]
CultureMech_008725 --> ing7_cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing7_cyanocobalamin -.-> CHEBI_17439
ing8_KBr["KBr"]
CultureMech_008725 --> ing8_KBr
CHEBI_32030["CHEBI:32030"]
ing8_KBr -.-> CHEBI_32030
ing9_SrCl2["SrCl2"]
CultureMech_008725 --> ing9_SrCl2
CHEBI_36383["CHEBI:36383"]
ing9_SrCl2 -.-> CHEBI_36383
ing10_Dry_yeast["Dry yeast"]
CultureMech_008725 --> ing10_Dry_yeast
ing11___glycerophosphate_2Na["β-glycerophosphate-2Na"]
CultureMech_008725 --> ing11___glycerophosphate_2Na
ing12_Bacto_Agar["Bacto Agar"]
CultureMech_008725 --> ing12_Bacto_Agar
ing13_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008725 --> ing13_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_2H2O -.-> CHEBI_75213
ing14_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008725 --> ing14_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_4H2O -.-> CHEBI_86368
ing15_ZnCl2["ZnCl2"]
CultureMech_008725 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_KI["KI"]
CultureMech_008725 --> ing16_KI
CHEBI_8346["CHEBI:8346"]
ing16_KI -.-> CHEBI_8346
ing17_CuSO4["CuSO4"]
CultureMech_008725 --> ing17_CuSO4
CHEBI_23414["CHEBI:23414"]
ing17_CuSO4 -.-> CHEBI_23414
ing18_CoCl2["CoCl2"]
CultureMech_008725 --> ing18_CoCl2
CHEBI_35696["CHEBI:35696"]
ing18_CoCl2 -.-> CHEBI_35696
ing19_SnCl2_2H2O["SnCl2·2H2O"]
CultureMech_008725 --> ing19_SnCl2_2H2O
CHEBI_78074["CHEBI:78074"]
ing19_SnCl2_2H2O -.-> CHEBI_78074
ing20_LiCl["LiCl"]
CultureMech_008725 --> ing20_LiCl
CHEBI_48607["CHEBI:48607"]
ing20_LiCl -.-> CHEBI_48607
ing21_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_008725 --> ing21_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing21_Fe_III__EDTA -.-> CHEBI_30729
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
H3BO3
H3BO3 61.833 g/mol
|
4 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
0.5 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 9 |
KBr
Br.K 119.002 g/mol
|
16 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
SrCl2
2Cl.Sr 158.526 g/mol
|
6 G_PER_L |
CHEBI:36383
strontium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Dry yeast | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 12 | β-glycerophosphate-2Na | 20 G_PER_L | Role: Mineral source; Properties: Organic compound, Defined component, Simple component | |
| 13 | Bacto Agar | 15 G_PER_L | Role: Solidifying component | |
| 14 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
10 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
20 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
KI
I.K 166.002 g/mol
|
20 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuSO4
Cu.O4S 159.609 g/mol
|
10 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CoCl2
2Cl.Co 129.839 g/mol
|
20 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
SnCl2·2H2O
2Cl.2H2O.Sn 225.647 g/mol
|
5 G_PER_L |
CHEBI:78074
tin(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
LiCl
Cl.Li 42.394 g/mol
|
5 G_PER_L |
CHEBI:48607
lithium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
8 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
Properties: Defined component, Organic compound, Simple component |
Solutions
Trace element solution*
Dose: 0.2 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_vy_2_agar (CultureMech:008725)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.136983Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2131 |
| 2026-01-28T19:48:26.752011Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:44.372988+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:14.588831Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:59.829497Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008725 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.473018+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "modified-VY/2-Agar" to "modified_vy_2_agar" for consistent matching |
| 2026-05-16T09:04:26.420257+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:38.005191+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:26.959752+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.026773+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:50.007186+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 70cc2b4fbe667e790ecaf62c33d2fc432e02df10e8c62c90906481bb6846e179 |