modified_biebl_and_pennings_medium (CultureMech:008745)

Overview

External ontology

TOGO:M2150 — Modified Biebl and Penning's Medium

Applications

Microbial cultivation

High-metal flag

True

Notes

Source: https://togomedium.org/medium/M2150 Original source: NBRC - NBRC_M1504 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1504

Composition

flowchart LR
    CultureMech_008745["modified_biebl_and_pennings_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008745 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008745 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008745 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_008745 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008745 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_KH2PO4["KH2PO4"]
    CultureMech_008745 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_008745 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_008745 --> ing7_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing7_Sodium_pyruvate -.-> CHEBI_50144
    ing8_Peptone["Peptone"]
    CultureMech_008745 --> ing8_Peptone
    ing9_N2["N2"]
    CultureMech_008745 --> ing9_N2
    CHEBI_17997["CHEBI:17997"]
    ing9_N2 -.-> CHEBI_17997
    ing10_Biotin["Biotin"]
    CultureMech_008745 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_008745 --> ing11_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing11_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing12_Thiamine_HCl["Thiamine-HCl"]
    CultureMech_008745 --> ing12_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing12_Thiamine_HCl -.-> CHEBI_49105
    ing13_Ca_pantothenate["Ca-pantothenate"]
    CultureMech_008745 --> ing13_Ca_pantothenate
    CHEBI_29032["CHEBI:29032"]
    ing13_Ca_pantothenate -.-> CHEBI_29032
    ing14_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_008745 --> ing14_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing14_Pyridoxine_HCl -.-> CHEBI_30961
    ing15_Folic_acid["Folic acid"]
    CultureMech_008745 --> ing15_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing15_Folic_acid -.-> CHEBI_27470
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_008745 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_Riboflavin["Riboflavin"]
    CultureMech_008745 --> ing17_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing17_Riboflavin -.-> CHEBI_17015
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_008745 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_008745 --> ing19_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_2H2O -.-> CHEBI_75213
    ing20_H3BO3["H3BO3"]
    CultureMech_008745 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_008745 --> ing21_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing21_MnCl2_4H2O -.-> CHEBI_86368
    ing22_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_008745 --> ing22_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing22_CoCl2_6H2O -.-> CHEBI_35696
    ing23_NiCl2_6H2O["NiCl2·6H2O"]
    CultureMech_008745 --> ing23_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_6H2O -.-> CHEBI_34887
    ing24_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_008745 --> ing24_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing24_CuCl2_2H2O -.-> CHEBI_86318
    ing25_ZnCl2["ZnCl2"]
    CultureMech_008745 --> ing25_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing25_ZnCl2 -.-> CHEBI_49976
    ing26_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
    CultureMech_008745 --> ing26_HCl__25___7_7_M_
    ing27_FeCl2_4H2O["FeCl2·4H2O"]
    CultureMech_008745 --> ing27_FeCl2_4H2O
    CHEBI_86249["CHEBI:86249"]
    ing27_FeCl2_4H2O -.-> CHEBI_86249

Ingredients (28)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	992.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 990.0]
2	MgSO4·7H2O 7H2O.Mg.O4S 246.473 g/mol	0.2 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3	Yeast extract	1 G_PER_L		Role: Nutrient source; Properties: Undefined component
4	NaCl Cl.Na 58.443 g/mol	0.4 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5	CaCl2·2H2O Ca.2Cl.2H2O 147.014 g/mol	0.05 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6	KH2PO4 H2O4P.K 136.084 g/mol	0.5 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
7	NH4Cl Cl.H4N 53.492 g/mol	0.6 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
8	Sodium pyruvate C3H3O3.Na 110.044 g/mol	3 G_PER_L	CHEBI:50144 sodium pyruvate	Role: Carbon source; Properties: Organic compound, Defined component, Simple component
9	Peptone	1 G_PER_L
10	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: Gas
11	Biotin C10H16N2O3S 244.316 g/mol	2 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
12	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	1 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
13	Thiamine-HCl C12H17N4OS.Cl.HCl 337.276 g/mol	5 G_PER_L	CHEBI:49105 thiamine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14	Ca-pantothenate C9H16NO5 218.229 g/mol	5 G_PER_L	CHEBI:29032 (R)-pantothenate	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
15	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	10 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
16	Folic acid C19H19N7O6 441.404 g/mol	2 G_PER_L	CHEBI:27470 folic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
17	Vitamin B12	0.01 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
18	Riboflavin C17H20N4O6 376.369 g/mol	5 G_PER_L	CHEBI:17015 riboflavin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
19	Nicotinic acid C6H5NO2 123.111 g/mol	5 G_PER_L	CHEBI:15940 nicotinic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
20	Na2MoO4·2H2O 2H2O.MoO4.2Na 241.946 g/mol	40 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21	H3BO3 H3BO3 61.833 g/mol	60 G_PER_L	CHEBI:33118 boric acid	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22	MnCl2·4H2O Cl2Mn.4H2O 197.904 g/mol	100 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
23	CoCl2·6H2O 2Cl.Co 129.839 g/mol	200 G_PER_L	CHEBI:35696 cobalt dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
24	NiCl2·6H2O Cl2Ni 129.599 g/mol	20 G_PER_L	CHEBI:34887 nickel dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
25	CuCl2·2H2O Cl2Cu.2H2O 170.482 g/mol	20 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
26	ZnCl2 2Cl.Zn 136.296 g/mol	70 G_PER_L	CHEBI:49976 zinc dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
27	HCl (25%; 7.7 M)	10 G_PER_L		Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
28	FeCl2·4H2O Cl2Fe.4H2O 198.811 g/mol	1.5 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component

Solutions

Ferric citrate solution* (0.1%)

Dose: 5 G_PER_L

Role: Mineral source; Properties: Defined component, Organic compound, Solution

Vitamin solution**

Dose: 2 G_PER_L

See mediadive_6241_Vitamin_solution.yaml for composition

Trace element solution SL7***

Dose: 1 G_PER_L

See mediadive_2739_Trace_element_solution_SL7.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

modified_biebl_and_pennings_medium (CultureMech:008745)

Timestamp	Curator	Action	Notes
2026-01-27T06:35:59.169786Z	togo-import	Imported from TOGO Medium	Source: NBRC, ID: M2150
2026-01-28T19:48:11.117069Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: N2
2026-03-09T08:04:18.217799+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 3 solution(s) from ingredients
2026-03-13T03:08:45.900765Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 2 duplicate ingredient(s)
2026-03-13T06:19:59.999942Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008745
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:48.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.652381+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Modified Biebl and Penning's Medium" to "modified_biebl_and_pennings_medium" for consistent matching
2026-05-16T09:04:25.164389+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:41:36.329293+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:48.721453+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24)	Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T06:58:25.813107+00:00	mgso4-heptahydrate-term-fix-v1.0	Corrected MgSO4·7H2O primary term grounding to heptahydrate	Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:08.557543+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:48.114865+00:00	merge_recipes.py	MERGED_RECIPES	merged 1 source recipe(s) on fingerprint 810773fad88c46fd4f3e42cb202b87ac7c7df4ca0f045a17a258ee1d10bb8458