Overview
- External ontology
- TOGO:M2152 — Modified M30 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2152 Original source: NBRC - NBRC_M1507 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1507
Composition
flowchart LR
CultureMech_008747["modified_m30_medium"]
ing0_0_1_M_Tris_HCl__pH7_5_["0.1 M Tris/HCl (pH7.5)"]
CultureMech_008747 --> ing0_0_1_M_Tris_HCl__pH7_5_
ing1_Distilled_water["Distilled water"]
CultureMech_008747 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_008747 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_Artificial_seawater["Artificial seawater"]
CultureMech_008747 --> ing3_Artificial_seawater
ing4_Glucose["Glucose"]
CultureMech_008747 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Agar["Agar"]
CultureMech_008747 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008747 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_008747 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
CultureMech_008747 --> ing8_Nitrilotriacetic_acid__NTA_
CHEBI_44557["CHEBI:44557"]
ing8_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
ing9__NH4_6MoO7O24_x_4_H2O["(NH4)6MoO7O24 x 4 H2O"]
CultureMech_008747 --> ing9__NH4_6MoO7O24_x_4_H2O
ing10_CaCl2_x_7_H2O["CaCl2 x 7 H2O"]
CultureMech_008747 --> ing10_CaCl2_x_7_H2O
CHEBI_3312["CHEBI:3312"]
ing10_CaCl2_x_7_H2O -.-> CHEBI_3312
ing11_KOH["KOH"]
CultureMech_008747 --> ing11_KOH
CHEBI_32035["CHEBI:32035"]
ing11_KOH -.-> CHEBI_32035
ing12_NaOH["NaOH"]
CultureMech_008747 --> ing12_NaOH
CHEBI_32145["CHEBI:32145"]
ing12_NaOH -.-> CHEBI_32145
ing13_NaCl["NaCl"]
CultureMech_008747 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_008747 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_008747 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_H3BO3["H3BO3"]
CultureMech_008747 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_008747 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_008747 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_008747 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_008747 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_ZnCl2["ZnCl2"]
CultureMech_008747 --> ing21_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing21_ZnCl2 -.-> CHEBI_49976
ing22_FeCl2_x_6_H2O["FeCl2 x 6 H2O"]
CultureMech_008747 --> ing22_FeCl2_x_6_H2O
CHEBI_30812["CHEBI:30812"]
ing22_FeCl2_x_6_H2O -.-> CHEBI_30812
ing23_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_008747 --> ing23_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing23_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing24_CuCl2_4H2O["CuCl2⋅4H2O"]
CultureMech_008747 --> ing24_CuCl2_4H2O
ing25_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_008747 --> ing25_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing25_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing26_N_acetylglucosamine["N-acetylglucosamine"]
CultureMech_008747 --> ing26_N_acetylglucosamine
CHEBI_59640["CHEBI:59640"]
ing26_N_acetylglucosamine -.-> CHEBI_59640
ing27_Biotin["Biotin"]
CultureMech_008747 --> ing27_Biotin
CHEBI_15956["CHEBI:15956"]
ing27_Biotin -.-> CHEBI_15956
ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008747 --> ing28_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
ing29_Pyridoxine_HCl__Vitamin_B6_["Pyridoxine-HCl (Vitamin B6)"]
CultureMech_008747 --> ing29_Pyridoxine_HCl__Vitamin_B6_
CultureMech_008747 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 0.1 M Tris/HCl (pH7.5) | 50 G_PER_L | Role: Buffer; Properties: Complex component, Defined component, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1172.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 180.0, 950.0, 1.0, 40.0, 1.0] |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 | Artificial seawater | 700 G_PER_L | Role: Solvating media, Mineral source; Properties: Defined component, Complex component, Solution | |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
99 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
22.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 10.0, 12.8] |
| 10 | (NH4)6MoO7O24 x 4 H2O | 9.3 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 11 |
CaCl2 x 7 H2O
Ca.2Cl 110.984 g/mol
|
3.3 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source |
| 12 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 13 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.024 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.12 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
FeCl2 x 6 H2O
Cl2Fe 126.751 g/mol
|
1.35 G_PER_L |
CHEBI:30812
iron dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.018 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 | CuCl2⋅4H2O | 0.025 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Simple component, Complex component | |
| 26 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.01 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 27 | N-acetylglucosamine | 2 G_PER_L |
CHEBI:59640
N-acetylglucosamine
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 28 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 | Pyridoxine-HCl (Vitamin B6) | 10 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 31 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 | Riboflavin (Vitamin B2) | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 34 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 | Thiamine-HCl⋅H2O (Vitamin B1) | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 36 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 37 |
D-Ca-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Defined component |
Solutions
Solution 1*
Dose: 20 G_PER_L
See mediadive_6108_Solution_1.yaml for composition
Solution 2***
Dose: 50 G_PER_L
See mediadive_6109_Solution_2.yaml for composition
Trace element solution**
Dose: 50 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Vitamin solution****
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_m30_medium (CultureMech:008747)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:59.174430Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2152 |
| 2026-01-28T19:48:40.709699Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: NaOH; Added default concentration for ingredient: NaOH; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:05:08.579636+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:40.314295Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:20:00.022566Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008747 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.674864+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified M30 Medium" to "modified_m30_medium" for consistent matching |
| 2026-05-16T09:04:25.557241+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:36.903754+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:26.171672+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.683780+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:48.611475+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9943eb5a2f1eba5facecec01fb1509d08075e6adc9b968b2712fe0ca235e1c68 |