modified_gam_broth_glucose_acetate

CultureMech:008755 bacterial COMPLEX LIQUID
Original name: Modified GAM broth + glucose & acetate

Overview

External ontology
TOGO:M2161 — Modified GAM broth + glucose & acetate
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2161 Original source: NBRC - NBRC_M1534 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1534

Composition

flowchart LR
    CultureMech_008755["modified_gam_broth_glucose_acetate"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008755 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Sodium_acetate["Sodium acetate"]
    CultureMech_008755 --> ing1_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing1_Sodium_acetate -.-> CHEBI_32954
    ing2_Glucose["Glucose"]
    CultureMech_008755 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_N2["N2"]
    CultureMech_008755 --> ing3_N2
    CHEBI_17997["CHEBI:17997"]
    ing3_N2 -.-> CHEBI_17997

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Sodium acetate
C2H3O2.Na 82.034 g/mol
2.7 G_PER_L CHEBI:32954
sodium acetate
Role: Buffer; Properties: Defined component, Organic compound, Simple component
3 Glucose
C6H12O6 180.156 g/mol
9.5 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Simple component, Organic compound, Defined component
4 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: Gas

Solutions

Nissui Modified GAM Broth*

Dose: 41.7 G_PER_L

Properties: Undefined component, Complex component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:59.188096Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2161
2026-01-28T19:48:27.974741Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:46.311167+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:00.074949Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008755
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:24.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:18.730553+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified GAM broth + glucose & acetate" to "modified_gam_broth_glucose_acetate" for consistent matching
2026-05-16T09:04:25.355748+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:36.613680+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:48.312520+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint f6d2371ff5874f4e8973fdf3a74939478cffbeeab3b63c95b59815d2ea3ccf1d