Overview
- External ontology
- TOGO:M2173 — Chloroflexus Aggregans Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2173 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium87a.pdf
Composition
flowchart LR
CultureMech_008767["chloroflexus_aggregans_medium"]
ing0_Medium_87["Medium 87"]
CultureMech_008767 --> ing0_Medium_87
ing1_N2_gas["N2 gas"]
CultureMech_008767 --> ing1_N2_gas
ing2_Distilled_water["Distilled water"]
CultureMech_008767 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008767 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Yeast_extract["Yeast extract"]
CultureMech_008767 --> ing4_Yeast_extract
ing5_NaCl["NaCl"]
CultureMech_008767 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_008767 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_NaNO3["NaNO3"]
CultureMech_008767 --> ing7_NaNO3
CHEBI_63005["CHEBI:63005"]
ing7_NaNO3 -.-> CHEBI_63005
ing8_KNO3["KNO3"]
CultureMech_008767 --> ing8_KNO3
CHEBI_63043["CHEBI:63043"]
ing8_KNO3 -.-> CHEBI_63043
ing9_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_008767 --> ing9_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing9_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing10_Glycyl_glycine["Glycyl-glycine"]
CultureMech_008767 --> ing10_Glycyl_glycine
CHEBI_17201["CHEBI:17201"]
ing10_Glycyl_glycine -.-> CHEBI_17201
ing11_Fe_III__citrate["Fe(III) citrate"]
CultureMech_008767 --> ing11_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing11_Fe_III__citrate -.-> CHEBI_144421
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_008767 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_008767 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_008767 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_008767 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_008767 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_008767 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_008767 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_008767 --> ing19_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing19_Na2S_x_9_H2O -.-> CHEBI_76209
ing20_Biotin["Biotin"]
CultureMech_008767 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008767 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Ca_pantothenate["Ca-pantothenate"]
CultureMech_008767 --> ing22_Ca_pantothenate
CHEBI_29032["CHEBI:29032"]
ing22_Ca_pantothenate -.-> CHEBI_29032
ing23_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_008767 --> ing23_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing23_Pyridoxine_HCl -.-> CHEBI_30961
ing24_Folic_acid["Folic acid"]
CultureMech_008767 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Vitamin_B12["Vitamin B12"]
CultureMech_008767 --> ing25_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing25_Vitamin_B12 -.-> CHEBI_176843
ing26_Nicotinic_acid["Nicotinic acid"]
CultureMech_008767 --> ing26_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing26_Nicotinic_acid -.-> CHEBI_15940
ing27_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_008767 --> ing27_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing27_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing28_Na3_EDTA["Na3-EDTA"]
CultureMech_008767 --> ing28_Na3_EDTA
CHEBI_64734["CHEBI:64734"]
ing28_Na3_EDTA -.-> CHEBI_64734
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Medium 87 | 90 G_PER_L | ||
| 2 | N2 gas | variable VARIABLE | Properties: ガス | |
| 3 |
Distilled water
H2O 18.015 g/mol
|
2350.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 1050.0, 100.0, 1000.0, 100.0, 100.0] |
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
0.1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NaNO3
NO3.Na 84.994 g/mol
|
0.5 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KNO3
K.NO3 101.102 g/mol
|
0.1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.1 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Glycyl-glycine
C4H8N2O3 132.119 g/mol
|
1 G_PER_L |
CHEBI:17201
glycylglycine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 12 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.1 G_PER_L |
CHEBI:144421
iron(III) citrate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
1.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
5 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
50 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
50 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
50 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
50 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
100 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
100 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Na3-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
200 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
Solutions
Neutralized sulfide solution
Dose: 1 G_PER_L
Vitamin solution CA
Dose: 1 G_PER_L
See mediadive_5560_Vitamin_solution_CA.yaml for composition
Fe(III) citrate solution
Dose: 5 G_PER_L
Trace element solution SL-6
Dose: 1 G_PER_L
See mediadive_25_Trace_element_solution_SL-6.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2173_Chloroflexus_Aggregans_Medium (CultureMech:008767)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.117786Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2173 |
| 2026-01-28T19:48:12.533076Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:04:20.712477+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:48.742972Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:20:00.165561Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008767 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.826960+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chloroflexus Aggregans Medium" to "chloroflexus_aggregans_medium" for consistent matching |
| 2026-05-16T09:03:12.449549+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:35.599937+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:31.823331+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:25.583855+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.642321+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:10.568107+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 59ae5520f1daea198db3a37a1cf1d7ac2ee0d5fa3419e08c8c5765151e3b934d |