Overview
- External ontology
- TOGO:M2186 — CENS medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2186 Original URL: https://www.atcc.org/~/media/077350E067794D9DB1954A2325A951D8.ashx
Composition
flowchart LR
CultureMech_008780["cens_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008780 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_008780 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_008780 --> ing2_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2___2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_008780 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_008780 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_008780 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Vitamin_B12["Vitamin B12"]
CultureMech_008780 --> ing6_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing6_Vitamin_B12 -.-> CHEBI_176843
ing7_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008780 --> ing7_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing7_Sodium_pyruvate -.-> CHEBI_50144
ing8_d_Biotin["d-Biotin"]
CultureMech_008780 --> ing8_d_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_d_Biotin -.-> CHEBI_15956
ing9_Na2S2O3___5H2O["Na2S2O3 . 5H2O"]
CultureMech_008780 --> ing9_Na2S2O3___5H2O
CHEBI_32150["CHEBI:32150"]
ing9_Na2S2O3___5H2O -.-> CHEBI_32150
ing10_Soytone__BD_243620_["Soytone (BD 243620)"]
CultureMech_008780 --> ing10_Soytone__BD_243620_
ing11_EDTA["EDTA"]
CultureMech_008780 --> ing11_EDTA
CHEBI_4735["CHEBI:4735"]
ing11_EDTA -.-> CHEBI_4735
ing12_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_008780 --> ing12_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4___2H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_008780 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_008780 --> ing14_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2___4H2O -.-> CHEBI_86368
ing15_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_008780 --> ing15_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2___6H2O -.-> CHEBI_35696
ing16_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_008780 --> ing16_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2___6H2O -.-> CHEBI_34887
ing17_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_008780 --> ing17_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2___2H2O -.-> CHEBI_86318
ing18_ZnCl2["ZnCl2"]
CultureMech_008780 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_Na2SeO3["Na2SeO3"]
CultureMech_008780 --> ing19_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing19_Na2SeO3 -.-> CHEBI_48843
ing20_NaVO3___nH2O__meta_["NaVO3 . nH2O (meta)"]
CultureMech_008780 --> ing20_NaVO3___nH2O__meta_
ing21_FeCl2___4H2O["FeCl2 . 4H2O"]
CultureMech_008780 --> ing21_FeCl2___4H2O
CHEBI_86249["CHEBI:86249"]
ing21_FeCl2___4H2O -.-> CHEBI_86249
ing22_Concentrated_HCl["Concentrated HCl"]
CultureMech_008780 --> ing22_Concentrated_HCl
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1151.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 250.0, 900.0] |
| 2 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
75 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Vitamin B12 | 20 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 8 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
2.2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 9 |
d-Biotin
C10H16N2O3S 244.316 g/mol
|
15 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 10 |
Na2S2O3 . 5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 11 | Soytone (BD 243620) | 4 G_PER_L | Properties: Undefined component | |
| 12 |
EDTA
C10H16N2O8 292.244 g/mol
|
9.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Organic compound, Simple component, Defined component [Merged 3 duplicates: 5.0, 2.5, 2.0] |
| 13 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.2 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
20 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
50 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
10 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
50 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
5 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 | NaVO3 . nH2O (meta) | 5 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 22 |
FeCl2 . 4H2O
Cl2Fe.4H2O 198.811 g/mol
|
1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 | Concentrated HCl | 3 G_PER_L | Role: Solvating media |
Solutions
True Blue Trace Elements
Dose: 1 G_PER_L
See mediadive_1533_True_Blue_Trace_Elements.yaml for composition
Chelated Iron Solution
Dose: 2 G_PER_L
See mediadive_4790_Chelated_iron_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2186_CENS_medium (CultureMech:008780)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.143259Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2186 |
| 2026-01-28T19:48:29.175120Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:48.418466+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:19.441446Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:00.272982Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008780 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:18.939150+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CENS medium" to "cens_medium" for consistent matching |
| 2026-05-16T09:03:12.507837+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.659084+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:25.634857+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.660226+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:10.638373+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 05c27241db91d04170624bae3ce379e867617c7e9cc9e56bbc267d478a4ba4a5 |