Overview
- External ontology
- TOGO:M221 — Methanococcus McC Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M221 Original source: JCM - JCM_M228 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=228
Composition
flowchart LR
CultureMech_008809["methanococcus_mcc_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008809 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_008809 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_K2HPO4["K2HPO4"]
CultureMech_008809 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Resazurin["Resazurin"]
CultureMech_008809 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Na2S_9H2O["Na2S・9H2O"]
CultureMech_008809 --> ing4_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_9H2O -.-> CHEBI_76209
ing5_NaHCO3["NaHCO3"]
CultureMech_008809 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Sodium_acetate["Sodium acetate"]
CultureMech_008809 --> ing6_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate -.-> CHEBI_32954
ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008809 --> ing7_Yeast_extract__BD_Difco_
ing8_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_008809 --> ing8_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008809 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_N2["N2"]
CultureMech_008809 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
ing11_Hydrogen_gas["Hydrogen gas"]
CultureMech_008809 --> ing11_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing11_Hydrogen_gas -.-> CHEBI_18276
ing12_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_008809 --> ing12_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_7H2O -.-> CHEBI_31795
ing13_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_008809 --> ing13_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_2H2O -.-> CHEBI_86158
ing14_NH4Cl["NH4Cl"]
CultureMech_008809 --> ing14_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing14_NH4Cl -.-> CHEBI_31206
ing15_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_008809 --> ing15_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing15_MgCl2_6H2O -.-> CHEBI_86345
ing16_KCl["KCl"]
CultureMech_008809 --> ing16_KCl
CHEBI_32588["CHEBI:32588"]
ing16_KCl -.-> CHEBI_32588
ing17_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_008809 --> ing17_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_2H2O -.-> CHEBI_75213
ing18_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_008809 --> ing18_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_6H2O -.-> CHEBI_34887
ing19_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_008809 --> ing19_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_7H2O -.-> CHEBI_32312
ing20_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_008809 --> ing20_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_5H2O -.-> CHEBI_31440
ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_008809 --> ing21_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
ing22_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_008809 --> ing22_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_2H2O -.-> CHEBI_63939
ing23_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_008809 --> ing23_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing23_MnSO4_xH2O -.-> CHEBI_86360
ing24_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2・6H2O"]
CultureMech_008809 --> ing24_Fe_NH4_2_SO4_2_6H2O
CHEBI_76181["CHEBI:76181"]
ing24_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
ing25_Na2SeO3["Na2SeO3"]
CultureMech_008809 --> ing25_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing25_Na2SeO3 -.-> CHEBI_48843
ing26_KOH["KOH"]
CultureMech_008809 --> ing26_KOH
CHEBI_32035["CHEBI:32035"]
ing26_KOH -.-> CHEBI_32035
ing27_HCl["HCl"]
CultureMech_008809 --> ing27_HCl
CHEBI_17883["CHEBI:17883"]
ing27_HCl -.-> CHEBI_17883
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
586.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 485.0, 1.0, 100.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
22 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 5 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
1.36 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 8 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 9 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 13 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.9 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.28 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
KCl
Cl.K 74.551 g/mol
|
0.67 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 23 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.1 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Fe(NH4)2(SO4)2・6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.4 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.2, 0.2] |
| 26 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.2 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 28 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
Solutions
General salts solution (see below)
Dose: 500 G_PER_L
Trace minerals solution (see below)
Dose: 10 G_PER_L
Iron stock solution (see below)
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M221_Methanococcus_McC_Medium (CultureMech:008809)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.643603Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M221 |
| 2026-01-28T19:48:11.367254Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: KOH; Added default concentration for ingredient: HCl |
| 2026-03-09T08:04:18.614307+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:46.333802Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:00.423453Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008809 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.097375+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanococcus McC Medium" to "methanococcus_mcc_medium" for consistent matching |
| 2026-05-16T09:03:12.588745+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:35.755952+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:25.710584+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.678949+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:26.215285+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d9f8f9cb8420fdda6bae402549ebec8f0de876bd410430e362ec2c4b9e6fd65f |