Overview
- External ontology
- TOGO:M2236 — Nitrobacter Medium 203
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2236 Original URL: https://www.atcc.org/~/media/EFEAE949B63E4C389AF65FC792391EB2.ashx
Composition
flowchart LR
CultureMech_008824["nitrobacter_medium_203"]
ing0_DI_Water["DI Water"]
CultureMech_008824 --> ing0_DI_Water
ing1_CaCl2["CaCl2"]
CultureMech_008824 --> ing1_CaCl2
CHEBI_3312["CHEBI:3312"]
ing1_CaCl2 -.-> CHEBI_3312
ing2_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_008824 --> ing2_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4___7H2O -.-> CHEBI_31795
ing3_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_008824 --> ing3_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4___7H2O -.-> CHEBI_75836
ing4_H2SO4__conc__["H2SO4 (conc.)"]
CultureMech_008824 --> ing4_H2SO4__conc__
ing5_EDTA["EDTA"]
CultureMech_008824 --> ing5_EDTA
CHEBI_4735["CHEBI:4735"]
ing5_EDTA -.-> CHEBI_4735
ing6_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_008824 --> ing6_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4___2H2O -.-> CHEBI_75213
ing7_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_008824 --> ing7_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2___4H2O -.-> CHEBI_86368
ing8_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_008824 --> ing8_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing8_CoCl2___6H2O -.-> CHEBI_35696
ing9_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_008824 --> ing9_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4___7H2O -.-> CHEBI_32312
ing10_CuSO4___5H2O["CuSO4 . 5H2O"]
CultureMech_008824 --> ing10_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing10_CuSO4___5H2O -.-> CHEBI_31440
ing11_NaNO2["NaNO2"]
CultureMech_008824 --> ing11_NaNO2
CHEBI_78870["CHEBI:78870"]
ing11_NaNO2 -.-> CHEBI_78870
ing12_K2HPO4["K2HPO4"]
CultureMech_008824 --> ing12_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing12_K2HPO4 -.-> CHEBI_131527
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | DI Water | 2500.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 7 duplicates: 1000.0, 100.0, 100.0, 100.0, 1000.0, 100.0, 100.0] | |
| 2 |
CaCl2
Ca.2Cl 110.984 g/mol
|
2 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 | H2SO4 (conc.) | 0.05 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Solution | |
| 6 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.14 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Organic compound, Simple component |
| 7 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.2 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.002 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CuSO4 . 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.02 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
NaNO2
NO2.Na 68.995 g/mol
|
41.4 G_PER_L |
CHEBI:78870
sodium nitrite
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.74 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 0.5 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 0.5 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 1 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Solution D
Dose: 0.5 G_PER_L
See mediadive_5313_Solution_D.yaml for composition
Solution E
Dose: 0.5 G_PER_L
See mediadive_4959_Solution_E.yaml for composition
Solution F
Dose: variable VARIABLE
See mediadive_6293_Solution_F.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- nitrobacter_medium_203 (CultureMech:008824)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.197865Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2236 |
| 2026-01-28T19:48:39.147157Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Solution F |
| 2026-03-09T08:05:05.778279+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:37.575784Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 6 duplicate ingredient(s) |
| 2026-03-13T06:20:00.544151Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008824 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.220392+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nitrobacter Medium 203" to "nitrobacter_medium_203" for consistent matching |
| 2026-05-16T09:04:27.654649+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:39.769678+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:28.203756+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.582196+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:51.404091+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 95fd6ad4433238e15c3fb9da61ade3ded95affdc27edf96b62b0af8dcb6016c4 |