cdm_lab_medium (CultureMech:008827)

Overview

External ontology

TOGO:M2239 — CDM-LAB medium

Applications

Microbial cultivation

High-metal flag

True

Notes

Source: https://togomedium.org/medium/M2239

Composition

flowchart LR
    CultureMech_008827["cdm_lab_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008827 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_KH2PO4["KH2PO4"]
    CultureMech_008827 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_K2HPO4["K2HPO4"]
    CultureMech_008827 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_p_aminobenzoic_acid["p-aminobenzoic acid"]
    CultureMech_008827 --> ing3_p_aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing3_p_aminobenzoic_acid -.-> CHEBI_30753
    ing4_Vitamin_B12["Vitamin B12"]
    CultureMech_008827 --> ing4_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing4_Vitamin_B12 -.-> CHEBI_176843
    ing5_Riboflavin["Riboflavin"]
    CultureMech_008827 --> ing5_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing5_Riboflavin -.-> CHEBI_17015
    ing6_Nicotinic_acid["Nicotinic acid"]
    CultureMech_008827 --> ing6_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing6_Nicotinic_acid -.-> CHEBI_15940
    ing7_CaCl2["CaCl2"]
    CultureMech_008827 --> ing7_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing7_CaCl2 -.-> CHEBI_3312
    ing8_CuSO4["CuSO4"]
    CultureMech_008827 --> ing8_CuSO4
    CHEBI_23414["CHEBI:23414"]
    ing8_CuSO4 -.-> CHEBI_23414
    ing9_FeCl3["FeCl3"]
    CultureMech_008827 --> ing9_FeCl3
    CHEBI_30808["CHEBI:30808"]
    ing9_FeCl3 -.-> CHEBI_30808
    ing10_MgCl2["MgCl2"]
    CultureMech_008827 --> ing10_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing10_MgCl2 -.-> CHEBI_6636
    ing11_Histidine["Histidine"]
    CultureMech_008827 --> ing11_Histidine
    CHEBI_27570["CHEBI:27570"]
    ing11_Histidine -.-> CHEBI_27570
    ing12_Asparagine["Asparagine"]
    CultureMech_008827 --> ing12_Asparagine
    CHEBI_22653["CHEBI:22653"]
    ing12_Asparagine -.-> CHEBI_22653
    ing13_Arginine["Arginine"]
    CultureMech_008827 --> ing13_Arginine
    CHEBI_32696["CHEBI:32696"]
    ing13_Arginine -.-> CHEBI_32696
    ing14_Ammonium_citrate["Ammonium citrate"]
    CultureMech_008827 --> ing14_Ammonium_citrate
    ing15_Tryptophan["Tryptophan"]
    CultureMech_008827 --> ing15_Tryptophan
    CHEBI_27897["CHEBI:27897"]
    ing15_Tryptophan -.-> CHEBI_27897
    ing16_Thiamine["Thiamine"]
    CultureMech_008827 --> ing16_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing16_Thiamine -.-> CHEBI_18385
    ing17_FeCl2["FeCl2"]
    CultureMech_008827 --> ing17_FeCl2
    CHEBI_30812["CHEBI:30812"]
    ing17_FeCl2 -.-> CHEBI_30812
    ing18_Cysteine["Cysteine"]
    CultureMech_008827 --> ing18_Cysteine
    CHEBI_15356["CHEBI:15356"]
    ing18_Cysteine -.-> CHEBI_15356
    ing19_Methionine["Methionine"]
    CultureMech_008827 --> ing19_Methionine
    ing20_Adenine_sulfate["Adenine sulfate"]
    CultureMech_008827 --> ing20_Adenine_sulfate
    ing21_Leucine["Leucine"]
    CultureMech_008827 --> ing21_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing21_Leucine -.-> CHEBI_15603
    ing22_Glycine["Glycine"]
    CultureMech_008827 --> ing22_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing22_Glycine -.-> CHEBI_15428
    ing23_MnCl2["MnCl2"]
    CultureMech_008827 --> ing23_MnCl2
    CHEBI_63041["CHEBI:63041"]
    ing23_MnCl2 -.-> CHEBI_63041
    ing24_Pantothenate["Pantothenate"]
    CultureMech_008827 --> ing24_Pantothenate
    CHEBI_16454["CHEBI:16454"]
    ing24_Pantothenate -.-> CHEBI_16454
    ing25_pyridoxamine_HCl["pyridoxamine-HCl"]
    CultureMech_008827 --> ing25_pyridoxamine_HCl
    CHEBI_131531["CHEBI:131531"]
    ing25_pyridoxamine_HCl -.-> CHEBI_131531
    ing26_Pyridoxine["Pyridoxine"]
    CultureMech_008827 --> ing26_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing26_Pyridoxine -.-> CHEBI_16709
    ing27_Uracil["Uracil"]
    CultureMech_008827 --> ing27_Uracil
    CHEBI_17568["CHEBI:17568"]
    ing27_Uracil -.-> CHEBI_17568
    ing28_Lipoic_acid["Lipoic acid"]
    CultureMech_008827 --> ing28_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_Lipoic_acid -.-> CHEBI_16494
    ing29_ZnSO4["ZnSO4"]
    CultureMech_008827 --> ing29_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing29_ZnSO4 -.-> CHEBI_35176
    CultureMech_008827 --> more["...25 more"]

Ingredients (55)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2	KH2PO4 H2O4P.K 136.084 g/mol	5 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
3	K2HPO4 HO4P.2K 174.174 g/mol	1 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
4	p-aminobenzoic acid C7H7NO2 137.138 g/mol	10 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
5	Vitamin B12	1 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
6	Riboflavin C17H20N4O6 376.369 g/mol	1 G_PER_L	CHEBI:17015 riboflavin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
7	Nicotinic acid C6H5NO2 123.111 g/mol	1 G_PER_L	CHEBI:15940 nicotinic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
8	CaCl2 Ca.2Cl 110.984 g/mol	50 G_PER_L	CHEBI:3312 calcium dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9	CuSO4 Cu.O4S 159.609 g/mol	2.5 G_PER_L	CHEBI:23414 copper(II) sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10	FeCl3 Cl3Fe 162.204 g/mol	3 G_PER_L	CHEBI:30808 iron trichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11	MgCl2 2Cl.Mg 95.211 g/mol	200 G_PER_L	CHEBI:6636 magnesium dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12	Histidine C6H9N3O2 155.157 g/mol	0.15 G_PER_L	CHEBI:27570 histidine	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
13	Asparagine C4H8N2O3 132.119 g/mol	0.1 G_PER_L	CHEBI:22653 asparagine	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
14	Arginine C6H15N4O2 175.212 g/mol	0.125 G_PER_L	CHEBI:32696 argininium(1+)	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
15	Ammonium citrate	0.6 G_PER_L		Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
16	Tryptophan C11H12N2O2 204.229 g/mol	0.05 G_PER_L	CHEBI:27897 tryptophan	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
17	Thiamine C12H17N4OS 265.362 g/mol	1 G_PER_L	CHEBI:18385 thiamine(1+)	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
18	FeCl2 Cl2Fe 126.751 g/mol	5 G_PER_L	CHEBI:30812 iron dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
19	Cysteine C3H7NO2S 121.161 g/mol	0.13 G_PER_L	CHEBI:15356 cysteine	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
20	Methionine	0.125 G_PER_L		Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
21	Adenine sulfate	35 G_PER_L		Role: Growth factor; Properties: Organic compound, Defined component, Simple component
22	Leucine C6H13NO2 131.175 g/mol	0.475 G_PER_L	CHEBI:15603 L-leucine	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
23	Glycine C2H5NO2 75.067 g/mol	0.175 G_PER_L	CHEBI:15428 glycine	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
24	MnCl2 Cl2Mn 125.844 g/mol	16 G_PER_L	CHEBI:63041 manganese(II) chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
25	Pantothenate C9H16NO5 218.229 g/mol	1 G_PER_L	CHEBI:16454 pantothenate	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
26	pyridoxamine-HCl C8H12N2O2.HCl 204.657 g/mol	5 G_PER_L	CHEBI:131531 pyridoxamine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
27	Pyridoxine C8H11NO3 169.18 g/mol	2 G_PER_L	CHEBI:16709 pyridoxine	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
28	Uracil C4H4N2O2 112.088 g/mol	22 G_PER_L	CHEBI:17568 uracil	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
29	Lipoic acid C8H14O2S2 206.332 g/mol	2.5 G_PER_L	CHEBI:16494 lipoic acid	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
30	ZnSO4 O4S.Zn 161.453 g/mol	5 G_PER_L	CHEBI:35176 zinc sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
31	Aspartic acid	0.42 G_PER_L		Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
32	Glutamic acid C5H9NO4 147.13 g/mol	0.5 G_PER_L	CHEBI:18237 glutamic acid	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
33	Glutamine	0.2 G_PER_L		Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
34	Isoleucine C6H13NO2 131.175 g/mol	0.21 G_PER_L	CHEBI:17191 L-isoleucine	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
35	Lysine	0.44 G_PER_L		Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
36	Phenylalanine C9H11NO2 165.192 g/mol	0.275 G_PER_L	CHEBI:17295 L-phenylalanine	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
37	Serine	0.34 G_PER_L		Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
38	Threonine	0.225 G_PER_L		Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
39	Tyrosine	0.25 G_PER_L		Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
40	Valine C5H11NO2 117.148 g/mol	0.325 G_PER_L	CHEBI:16414 L-valine	Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
41	L-ascorbic acid C6H8O6 176.124 g/mol	0.5 G_PER_L	CHEBI:29073 L-ascorbic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
42	Glucose C6H12O6 180.156 g/mol	10 G_PER_L	CHEBI:17234 glucose	Role: Carbon source; Properties: Organic compound, Defined component, Simple component
43	Thymidine C10H14N2O5 242.231 g/mol	5 G_PER_L	CHEBI:17748 thymidine	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
44	Acetate C2H3O2 59.044 g/mol	1 G_PER_L	CHEBI:30089 acetate	Role: Carbon source; Properties: Organic compound, Defined component, Simple component
45	CoSO4 Co.O4S 154.996 g/mol	2.5 G_PER_L	CHEBI:53470 cobalt(2+) sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
46	D-biotin C10H16N2O3S 244.316 g/mol	2.5 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
47	(NH4)6Mo7O24 2H4N.MoO4 196.014 g/mol	2.5 G_PER_L	CHEBI:91249 ammonium molybdate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
48	Guanine C5H5N5O 151.129 g/mol	27 G_PER_L	CHEBI:16235 guanine	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
49	Xanthine C5H4N4O2 152.111 g/mol	50 G_PER_L	CHEBI:15318 xanthine	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
50	Inosine C10H12N4O5 268.229 g/mol	5 G_PER_L	CHEBI:17596 inosine	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
51	Orotic acid C5H4N2O4 156.097 g/mol	5 G_PER_L	CHEBI:16742 orotic acid	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
52	Cystine	50 G_PER_L		Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
53	Alanine C3H7NO2 89.094 g/mol	0.24 G_PER_L	CHEBI:16977 L-alanine	Role: Nitrogen source
54	Proline C5H9NO2 115.132 g/mol	0.675 G_PER_L	CHEBI:26271 proline	Role: Nitrogen source
55	MOPS buffer C7H15NO4S 209.267 g/mol	variable VARIABLE	CHEBI:44115 3-(N-morpholino)propanesulfonic acid	Role: Buffer; Properties: Defined component, Organic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

cdm_lab_medium (CultureMech:008827)

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.203957Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2239
2026-01-28T19:48:36.009793Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: MOPS buffer
2026-02-06T05:10:46.923831Z	invalid-chebi-removal	Removed 1 invalid CHEBI ID(s)	Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:20:00.577985Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008827
2026-03-17T06:30:42.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.254009+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "CDM-LAB medium" to "cdm_lab_medium" for consistent matching
2026-05-16T09:03:26.934913+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:40:52.775734+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.376117+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:26.927846+00:00	merge_recipes.py	MERGED_RECIPES	merged 1 source recipe(s) on fingerprint 7f9c813fb83602092e7811470a43898ba38229d30aaf1328086323909f7fac6c