Overview
- External ontology
- TOGO:M2241 — Medium DH
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2241
Composition
flowchart LR
CultureMech_008830["medium_dh"]
ing0_HEPES_buffer["HEPES buffer"]
CultureMech_008830 --> ing0_HEPES_buffer
CHEBI_46756["CHEBI:46756"]
ing0_HEPES_buffer -.-> CHEBI_46756
ing1_Medium_D["Medium D"]
CultureMech_008830 --> ing1_Medium_D
ing2_Double_distilled_water["Double distilled water"]
CultureMech_008830 --> ing2_Double_distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Double_distilled_water -.-> CHEBI_15377
ing3_MgSO4_7H2O["MgSO4.7H2O"]
CultureMech_008830 --> ing3_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_7H2O -.-> CHEBI_31795
ing4_NaCl["NaCl"]
CultureMech_008830 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_NaNO3["NaNO3"]
CultureMech_008830 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_008830 --> ing6_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing6_Nitrilotriacetic_acid -.-> CHEBI_44557
ing7_KNO3["KNO3"]
CultureMech_008830 --> ing7_KNO3
CHEBI_63043["CHEBI:63043"]
ing7_KNO3 -.-> CHEBI_63043
ing8_Na2HPO4["Na2HPO4"]
CultureMech_008830 --> ing8_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing8_Na2HPO4 -.-> CHEBI_34683
ing9_CaSO4_2H2O["CaSO4.2H2O"]
CultureMech_008830 --> ing9_CaSO4_2H2O
CHEBI_32583["CHEBI:32583"]
ing9_CaSO4_2H2O -.-> CHEBI_32583
ing10_1M_NaOH["1M NaOH"]
CultureMech_008830 --> ing10_1M_NaOH
ing11_Distilled_water["Distilled water"]
CultureMech_008830 --> ing11_Distilled_water
ing11_Distilled_water -.-> CHEBI_15377
ing12_Na2MoO4_2H2O["Na2MoO4.2H2O"]
CultureMech_008830 --> ing12_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_2H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_008830 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_CoCl2_6H2O["CoCl2.6H2O"]
CultureMech_008830 --> ing14_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing14_CoCl2_6H2O -.-> CHEBI_35696
ing15_ZnSO4_7H2O["ZnSO4.7H2O"]
CultureMech_008830 --> ing15_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_7H2O -.-> CHEBI_32312
ing16_CuSO4_5H2O["CuSO4.5H2O"]
CultureMech_008830 --> ing16_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing16_CuSO4_5H2O -.-> CHEBI_31440
ing17_MnSO4_H2O["MnSO4.H2O"]
CultureMech_008830 --> ing17_MnSO4_H2O
CHEBI_86364["CHEBI:86364"]
ing17_MnSO4_H2O -.-> CHEBI_86364
ing18_H2SO4__concd_["H2SO4 (concd)"]
CultureMech_008830 --> ing18_H2SO4__concd_
ing19_FeCl3["FeCl3"]
CultureMech_008830 --> ing19_FeCl3
CHEBI_30808["CHEBI:30808"]
ing19_FeCl3 -.-> CHEBI_30808
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | HEPES buffer | 1.2 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Defined component, Solution |
| 2 | Medium D | 1000 G_PER_L | ||
| 3 |
Double distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.008 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
0.689 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.1 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 8 |
KNO3
K.NO3 101.102 g/mol
|
0.103 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.111 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CaSO4.2H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.06 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 | 1M NaOH | variable VARIABLE | Properties: Defined component, Inorganic compound, Solution | |
| 12 |
Distilled water
H2O 18.015 g/mol
|
2000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1000.0, 1000.0] |
| 13 |
Na2MoO4.2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.025 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.5 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CoCl2.6H2O
2Cl.Co 129.839 g/mol
|
0.045 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
ZnSO4.7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.5 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CuSO4.5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.025 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
MnSO4.H2O
H2O.Mn.O4S 169.016 g/mol
|
2.28 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 | H2SO4 (concd) | 0.5 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Solution | |
| 20 |
FeCl3
Cl3Fe 162.204 g/mol
|
0.2905 G_PER_L |
CHEBI:30808
iron trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Micronutrient solution
Dose: 0.5 G_PER_L
See mediadive_6236_Micronutrient_solution.yaml for composition
FeCl3 solution
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_dh (CultureMech:008830)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:56.210714Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2241 |
| 2026-01-28T19:48:30.929096Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 1M NaOH |
| 2026-03-09T08:04:51.451141+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:22.801788Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:00.609541Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008830 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.286682+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium DH" to "medium_dh" for consistent matching |
| 2026-05-16T09:04:19.796876+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:28.785712+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:05.947452+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.948411+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 129f0039040eeacccc7a5b12c3c369d55f772d5f47849a9242321d49d569b2c1 |