mrs_broth_oxoid

CultureMech:008831 bacterial COMPLEX LIQUID
Original name: MRS broth (Oxoid)

Overview

External ontology
TOGO:M2242 — MRS broth (Oxoid)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2242

Composition

flowchart LR
    CultureMech_008831["mrs_broth_oxoid"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008831 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Magnesium_sulphate_7H2O["Magnesium sulphate 7H2O"]
    CultureMech_008831 --> ing1_Magnesium_sulphate_7H2O
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008831 --> ing2_Yeast_extract
    ing3_Dipotassium_hydrogen_phosphate["Dipotassium hydrogen phosphate"]
    CultureMech_008831 --> ing3_Dipotassium_hydrogen_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing3_Dipotassium_hydrogen_phosphate -.-> CHEBI_131527
    ing4_Manganese_sulphate_4H2O["Manganese sulphate 4H2O"]
    CultureMech_008831 --> ing4_Manganese_sulphate_4H2O
    ing5_Sorbitan_mono_oleate["Sorbitan mono-oleate"]
    CultureMech_008831 --> ing5_Sorbitan_mono_oleate
    ing6_Triammonium_citrate["Triammonium citrate"]
    CultureMech_008831 --> ing6_Triammonium_citrate
    CHEBI_63037["CHEBI:63037"]
    ing6_Triammonium_citrate -.-> CHEBI_63037
    ing7_Sodium_acetate_3H2O["Sodium acetate 3H2O"]
    CultureMech_008831 --> ing7_Sodium_acetate_3H2O
    ing8_Glucose["Glucose"]
    CultureMech_008831 --> ing8_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing8_Glucose -.-> CHEBI_17234
    ing9__Lab_Lemco__powder["`Lab-Lemco’ powder"]
    CultureMech_008831 --> ing9__Lab_Lemco__powder
    ing10_Peptone["Peptone"]
    CultureMech_008831 --> ing10_Peptone

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 Magnesium sulphate 7H2O 0.2 G_PER_L Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 Yeast extract 4 G_PER_L Role: Nutrient source; Properties: Undefined component
4 Dipotassium hydrogen phosphate
HO4P.2K 174.174 g/mol
2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
5 Manganese sulphate 4H2O 0.05 G_PER_L Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 Sorbitan mono-oleate 1 G_PER_L Role: Carbon source; Properties: Simple component, Organic compound, Defined component
7 Triammonium citrate
C6H5O7.3H4N 243.216 g/mol
2 G_PER_L CHEBI:63037
triammonium citrate
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
8 Sodium acetate 3H2O 5 G_PER_L Role: Buffer; Properties: Simple component, Organic compound, Defined component
9 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Organic compound, Simple component, Defined component
10 `Lab-Lemco’ powder 8 G_PER_L Properties: Undefined component
11 Peptone 10 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.213301Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2242
2026-01-28T19:48:22.680227Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:00.617579Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008831
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.295112+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS broth (Oxoid)" to "mrs_broth_oxoid" for consistent matching
2026-06-05T07:41:38.448121+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:50.450058+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 99da356ab6115aafb4541825c3bd40988131f82cbfaf038630a1840403ca59c1