Overview
- External ontology
- TOGO:M2243 — Half Strength Bacto Marine Broth
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2243 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514a.pdf
Composition
flowchart LR
CultureMech_008832["half_strength_bacto_marine_broth"]
ing0_Distilled_water["Distilled water"]
CultureMech_008832 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Difco_Bacto_Marine_Broth__DIFCO_2216_["Difco Bacto Marine Broth (DIFCO 2216)"]
CultureMech_008832 --> ing1_Difco_Bacto_Marine_Broth__DIFCO_2216_
ing2_NaCl["NaCl"]
CultureMech_008832 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_H3BO3["H3BO3"]
CultureMech_008832 --> ing3_H3BO3
CHEBI_33118["CHEBI:33118"]
ing3_H3BO3 -.-> CHEBI_33118
ing4_KCl["KCl"]
CultureMech_008832 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaHCO3["NaHCO3"]
CultureMech_008832 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Na2SO4["Na2SO4"]
CultureMech_008832 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_CaCl2["CaCl2"]
CultureMech_008832 --> ing7_CaCl2
CHEBI_3312["CHEBI:3312"]
ing7_CaCl2 -.-> CHEBI_3312
ing8_Na2HPO4["Na2HPO4"]
CultureMech_008832 --> ing8_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing8_Na2HPO4 -.-> CHEBI_34683
ing9__NH4_NO3["(NH4)NO3"]
CultureMech_008832 --> ing9__NH4_NO3
CHEBI_63038["CHEBI:63038"]
ing9__NH4_NO3 -.-> CHEBI_63038
ing10_NaF["NaF"]
CultureMech_008832 --> ing10_NaF
CHEBI_28741["CHEBI:28741"]
ing10_NaF -.-> CHEBI_28741
ing11_KBr["KBr"]
CultureMech_008832 --> ing11_KBr
CHEBI_32030["CHEBI:32030"]
ing11_KBr -.-> CHEBI_32030
ing12_Fe_III__citrate["Fe(III) citrate"]
CultureMech_008832 --> ing12_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing12_Fe_III__citrate -.-> CHEBI_144421
ing13_SrCl2["SrCl2"]
CultureMech_008832 --> ing13_SrCl2
CHEBI_36383["CHEBI:36383"]
ing13_SrCl2 -.-> CHEBI_36383
ing14_Na_silicate["Na-silicate"]
CultureMech_008832 --> ing14_Na_silicate
CHEBI_60720["CHEBI:60720"]
ing14_Na_silicate -.-> CHEBI_60720
ing15_MgCl2__anhydrous_["MgCl2 (anhydrous)"]
CultureMech_008832 --> ing15_MgCl2__anhydrous_
ing16_Bacto_yeast_extract["Bacto yeast extract"]
CultureMech_008832 --> ing16_Bacto_yeast_extract
ing17_Bacto_peptone["Bacto peptone"]
CultureMech_008832 --> ing17_Bacto_peptone
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 | Difco Bacto Marine Broth (DIFCO 2216) | 18.7 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
19.45 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
H3BO3
H3BO3 61.833 g/mol
|
22 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.55 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.24 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1.8 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
8 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
(NH4)NO3
H4N.NO3 80.043 g/mol
|
1.6 G_PER_L |
CHEBI:63038
ammonium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NaF
F.Na 41.988 g/mol
|
2.4 G_PER_L |
CHEBI:28741
sodium fluoride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
KBr
Br.K 119.002 g/mol
|
0.08 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.1 G_PER_L |
CHEBI:144421
iron(III) citrate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 14 |
SrCl2
2Cl.Sr 158.526 g/mol
|
34 G_PER_L |
CHEBI:36383
strontium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na-silicate
2Na.O3Si 122.063 g/mol
|
4 G_PER_L |
CHEBI:60720
sodium silicate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 | MgCl2 (anhydrous) | 5.9 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 17 | Bacto yeast extract | 1 G_PER_L | Properties: Undefined component | |
| 18 | Bacto peptone | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2243_Half_Strength_Bacto_Marine_Broth (CultureMech:008832)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.214865Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2243 |
| 2026-01-28T19:48:20.521195Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.626121Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008832 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.303561+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Half Strength Bacto Marine Broth" to "half_strength_bacto_marine_broth" for consistent matching |
| 2026-06-05T07:40:35.820058+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.707596+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:10.775302+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 97a9d5b6ca059698b41a7005d2b6dc32d5480f67e2a14fa1aa010069ae0c346f |