Overview
- External ontology
- TOGO:M2247 — BHI agar with 5% bovine blood
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2247 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008835["bhi_agar_with_5_bovine_blood"]
ing0_Bovine_blood["Bovine blood"]
CultureMech_008835 --> ing0_Bovine_blood
ing1_Calf_brains["Calf brains"]
CultureMech_008835 --> ing1_Calf_brains
ing2_Beef_heart["Beef heart"]
CultureMech_008835 --> ing2_Beef_heart
ing3_Proteose_peptone["Proteose peptone"]
CultureMech_008835 --> ing3_Proteose_peptone
ing4_Dextrose["Dextrose"]
CultureMech_008835 --> ing4_Dextrose
CHEBI_17634["CHEBI:17634"]
ing4_Dextrose -.-> CHEBI_17634
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_008835 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_Disodium_phosphate["Disodium phosphate"]
CultureMech_008835 --> ing6_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing6_Disodium_phosphate -.-> CHEBI_34683
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bovine blood | 5 PERCENT_W_V | Role: Nutrient source | |
| 2 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 3 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 4 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 5 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 7 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- bhi_agar_with_5_bovine_blood (CultureMech:008835)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.218888Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2247 |
| 2026-03-13T06:20:00.638796Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008835 |
| 2026-03-17T19:46:19.316772+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BHI agar with 5% bovine blood" to "bhi_agar_with_5_bovine_blood" for consistent matching |
| 2026-08-06T03:05:25.113342+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d4ab3e23782cae2622d391a8297bac6c39524d7d519dd5d2ea8edc177e7a0a5c |