Overview
- External ontology
- TOGO:M2250 — Lactobacilli MRS Agar/Broth
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2250 Original URL: https://www.atcc.org/~/media/39839AC0CB884212A57DBF6936371D91.ashx
Composition
flowchart LR
CultureMech_008839["lactobacilli_mrs_agar_broth"]
ing0_DI_Water["DI Water"]
CultureMech_008839 --> ing0_DI_Water
ing1_Lactobacilli_MRS["Lactobacilli MRS"]
CultureMech_008839 --> ing1_Lactobacilli_MRS
ing2_Yeast_Extract["Yeast Extract"]
CultureMech_008839 --> ing2_Yeast_Extract
ing3_Dextrose["Dextrose"]
CultureMech_008839 --> ing3_Dextrose
CHEBI_17634["CHEBI:17634"]
ing3_Dextrose -.-> CHEBI_17634
ing4_Sodium_Acetate["Sodium Acetate"]
CultureMech_008839 --> ing4_Sodium_Acetate
CHEBI_32954["CHEBI:32954"]
ing4_Sodium_Acetate -.-> CHEBI_32954
ing5_Na2HPO4["Na2HPO4"]
CultureMech_008839 --> ing5_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing5_Na2HPO4 -.-> CHEBI_34683
ing6_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_008839 --> ing6_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing6_MnSO4_x_H2O -.-> CHEBI_86364
ing7_Sorbitan_Monooleate["Sorbitan Monooleate"]
CultureMech_008839 --> ing7_Sorbitan_Monooleate
ing8_Ammonium_Citrate["Ammonium Citrate"]
CultureMech_008839 --> ing8_Ammonium_Citrate
ing9_Beef_Extract["Beef Extract"]
CultureMech_008839 --> ing9_Beef_Extract
ing10_Proteose_Peptone__3["Proteose Peptone #3"]
CultureMech_008839 --> ing10_Proteose_Peptone__3
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | DI Water | 1000 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component | |
| 2 | Lactobacilli MRS | 55 G_PER_L | ||
| 3 | Yeast Extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
Dextrose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 5 |
Sodium Acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 6 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
2 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.05 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Sorbitan Monooleate | 1 G_PER_L | Role: Carbon source; Properties: Defined component, Organic compound, Simple component | |
| 9 | Ammonium Citrate | 2 G_PER_L | Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component | |
| 10 | Beef Extract | 10 G_PER_L | Properties: Undefined component | |
| 11 | Proteose Peptone #3 | 10 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2250_Lactobacilli_MRS_Agar_Broth (CultureMech:008839)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.222167Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2250 |
| 2026-01-28T19:48:36.695691Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:00.667463Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008839 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.344577+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Lactobacilli MRS Agar/Broth" to "lactobacilli_mrs_agar_broth" for consistent matching |
| 2026-06-05T07:40:35.847737+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:31.902930+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:10.794074+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 026713987ec69c93ec4c2aa329f3c93625d7f16b13dcecb211e5f4d4f2a1c070 |