nitrogen_free_sm_medium

CultureMech:008840 bacterial COMPLEX SOLID_AGAR
Original name: Nitrogen-free SM medium

Overview

External ontology
TOGO:M2251 — Nitrogen-free SM medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2251

Composition

flowchart LR
    CultureMech_008840["nitrogen_free_sm_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008840 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4.7H2O"]
    CultureMech_008840 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_008840 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008840 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_K2HPO4["K2HPO4"]
    CultureMech_008840 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Na2MoO4_2H2O["Na2MoO4.2H2O"]
    CultureMech_008840 --> ing5_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing5_Na2MoO4_2H2O -.-> CHEBI_75213
    ing6_Biotin["Biotin"]
    CultureMech_008840 --> ing6_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing6_Biotin -.-> CHEBI_15956
    ing7_CaCl2["CaCl2"]
    CultureMech_008840 --> ing7_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing7_CaCl2 -.-> CHEBI_3312
    ing8_MnSO4_H2O["MnSO4.H2O"]
    CultureMech_008840 --> ing8_MnSO4_H2O
    CHEBI_86364["CHEBI:86364"]
    ing8_MnSO4_H2O -.-> CHEBI_86364
    ing9_KOH["KOH"]
    CultureMech_008840 --> ing9_KOH
    CHEBI_32035["CHEBI:32035"]
    ing9_KOH -.-> CHEBI_32035
    ing10_DL_malic_acid["DL-malic acid"]
    CultureMech_008840 --> ing10_DL_malic_acid
    CHEBI_6650["CHEBI:6650"]
    ing10_DL_malic_acid -.-> CHEBI_6650
    ing11_Agar["Agar"]
    CultureMech_008840 --> ing11_Agar
    CHEBI_2509["CHEBI:2509"]
    ing11_Agar -.-> CHEBI_2509
    ing12_Fe_III__EDTA__0_66___wt_vol__in_water_["Fe(III)-EDTA (0.66% [wt/vol] in water)"]
    CultureMech_008840 --> ing12_Fe_III__EDTA__0_66___wt_vol__in_water_
    ing13_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_008840 --> ing13_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing13_Fe_III__EDTA -.-> CHEBI_30729

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
101.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 1.0, 100.0]
2 MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 NaCl
Cl.Na 58.443 g/mol
0.1 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.6 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 K2HPO4
HO4P.2K 174.174 g/mol
0.4 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
6 Na2MoO4.2H2O
2H2O.MoO4.2Na 241.946 g/mol
0.002 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
7 Biotin
C10H16N2O3S 244.316 g/mol
0.1 G_PER_L CHEBI:15956
biotin
 Role: Vitamin; Properties: Simple component, Organic compound, Defined component
8 CaCl2
Ca.2Cl 110.984 g/mol
0.02 G_PER_L CHEBI:3312
calcium dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 MnSO4.H2O
H2O.Mn.O4S 169.016 g/mol
0.01 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 KOH
HO.K 56.105 g/mol
4.5 G_PER_L CHEBI:32035
potassium hydroxide
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
11 DL-malic acid
C4H6O5 134.087 g/mol
5 G_PER_L CHEBI:6650
malic acid
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
12 Agar 2 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
13 Fe(III)-EDTA (0.66% [wt/vol] in water) 10 G_PER_L
14 Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
0.66 G_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
 Properties: Defined component, Organic compound, Simple component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.223655Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2251
2026-01-28T19:48:36.453574Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:32.594606Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:00.674946Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008840
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.352132+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Nitrogen-free SM medium" to "nitrogen_free_sm_medium" for consistent matching
2026-06-05T07:41:39.785585+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.589433+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:51.416683+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint a2e618a4a247e2621007aaf3aeed50e5d3065fdcd00c6f1136f690b653b73384