Overview
- External ontology
- TOGO:M2262 — PCR-S11 medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2262
Composition
flowchart LR
CultureMech_008849["pcr_s11_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_008849 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_Vitamin_B12["Vitamin B12"]
CultureMech_008849 --> ing1_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing1_Vitamin_B12 -.-> CHEBI_176843
ing2_Seawater__autoclaved_["Seawater (autoclaved)"]
CultureMech_008849 --> ing2_Seawater__autoclaved_
ing3_HEPES_NaOH__pH_7_5_["HEPES-NaOH (pH 7.5)"]
CultureMech_008849 --> ing3_HEPES_NaOH__pH_7_5_
ing4_Trace_metals__Gaffron__Se_["Trace metals 'Gaffron+Se'"]
CultureMech_008849 --> ing4_Trace_metals__Gaffron__Se_
ing5_Na_PO4__pH_7_5_["Na-PO4 (pH 7.5)"]
CultureMech_008849 --> ing5_Na_PO4__pH_7_5_
ing6_Na2_EDTA_FeCl3["Na2-EDTA/FeCl3"]
CultureMech_008849 --> ing6_Na2_EDTA_FeCl3
ing7_Distilled_water["Distilled water"]
CultureMech_008849 --> ing7_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing7_Distilled_water -.-> CHEBI_15377
ing8_Hepes["Hepes"]
CultureMech_008849 --> ing8_Hepes
CHEBI_46756["CHEBI:46756"]
ing8_Hepes -.-> CHEBI_46756
ing9_H3BO3["H3BO3"]
CultureMech_008849 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_NiCl2_6H2O["NiCl2.6H2O"]
CultureMech_008849 --> ing10_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_6H2O -.-> CHEBI_34887
ing11_ZnSO4_7H2O["ZnSO4.7H2O"]
CultureMech_008849 --> ing11_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_7H2O -.-> CHEBI_32312
ing12_CuSO4_5H2O["CuSO4.5H2O"]
CultureMech_008849 --> ing12_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_5H2O -.-> CHEBI_31440
ing13_KAI_SO4_2_12H2O["KAI(SO4)2.12H2O"]
CultureMech_008849 --> ing13_KAI_SO4_2_12H2O
ing14_Na2WO4_2H2O["Na2WO4.2H2O"]
CultureMech_008849 --> ing14_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing14_Na2WO4_2H2O -.-> CHEBI_63939
ing15_MnSO4_H2O["MnSO4.H2O"]
CultureMech_008849 --> ing15_MnSO4_H2O
CHEBI_86364["CHEBI:86364"]
ing15_MnSO4_H2O -.-> CHEBI_86364
ing16_KI["KI"]
CultureMech_008849 --> ing16_KI
CHEBI_8346["CHEBI:8346"]
ing16_KI -.-> CHEBI_8346
ing17_KBr["KBr"]
CultureMech_008849 --> ing17_KBr
CHEBI_32030["CHEBI:32030"]
ing17_KBr -.-> CHEBI_32030
ing18_Co_NO3_2_6H2O["Co(NO3)2.6H2O"]
CultureMech_008849 --> ing18_Co_NO3_2_6H2O
CHEBI_86214["CHEBI:86214"]
ing18_Co_NO3_2_6H2O -.-> CHEBI_86214
ing19__NH4_6Mo7O24_4H2O["(NH4)6Mo7O24.4H2O"]
CultureMech_008849 --> ing19__NH4_6Mo7O24_4H2O
CHEBI_91249["CHEBI:91249"]
ing19__NH4_6Mo7O24_4H2O -.-> CHEBI_91249
ing20_VSO5_5H2O["VSO5.5H2O"]
CultureMech_008849 --> ing20_VSO5_5H2O
ing21_Cr_NO3_3_9H2O["Cr(NO3)3.9H2O"]
CultureMech_008849 --> ing21_Cr_NO3_3_9H2O
ing22_SeO2["SeO2"]
CultureMech_008849 --> ing22_SeO2
ing23_Cd_NO3__4H2O["Cd(NO3).4H2O"]
CultureMech_008849 --> ing23_Cd_NO3__4H2O
ing24_50mM_NaH2PO4["50mM NaH2PO4"]
CultureMech_008849 --> ing24_50mM_NaH2PO4
ing25_50mM_Na2HPO4["50mM Na2HPO4"]
CultureMech_008849 --> ing25_50mM_Na2HPO4
ing26_NaH2PO4["NaH2PO4"]
CultureMech_008849 --> ing26_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing26_NaH2PO4 -.-> CHEBI_37585
ing27_Na2HPO4["Na2HPO4"]
CultureMech_008849 --> ing27_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing27_Na2HPO4 -.-> CHEBI_34683
ing28_0_1N_HCl["0.1N HCl"]
CultureMech_008849 --> ing28_0_1N_HCl
ing29_FeCl3["FeCl3"]
CultureMech_008849 --> ing29_FeCl3
CHEBI_30808["CHEBI:30808"]
ing29_FeCl3 -.-> CHEBI_30808
CultureMech_008849 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
400 MICROMOLAR |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 2 | Vitamin B12 | 10 MICROG_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 3 | Seawater (autoclaved) | 1000 G_PER_L | Role: Solvating media, Mineral source; Properties: Undefined component | |
| 4 | HEPES-NaOH (pH 7.5) | 1 MILLIMOLAR | ||
| 5 | Trace metals 'Gaffron+Se' | 0.5 G_PER_L | ||
| 6 | Na-PO4 (pH 7.5) | 50 MICROMOLAR | ||
| 7 | Na2-EDTA/FeCl3 | 8 MICROMOLAR | ||
| 8 |
Distilled water
H2O 18.015 g/mol
|
1004.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 500.0, 1.0, 1.0, 500.0, 2.0] |
| 9 | Hepes | 119.15 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
300 MICROMOLAR |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NiCl2.6H2O
Cl2Ni 129.599 g/mol
|
3 MICROMOLAR |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
ZnSO4.7H2O
7H2O.O4S.Zn 287.558 g/mol
|
6 MICROMOLAR |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuSO4.5H2O
Cu.5H2O.O4S 249.684 g/mol
|
3 MICROMOLAR |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 | KAI(SO4)2.12H2O | 6 MICROMOLAR | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 15 |
Na2WO4.2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.6 MICROMOLAR |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnSO4.H2O
H2O.Mn.O4S 169.016 g/mol
|
60 MICROMOLAR |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
KI
I.K 166.002 g/mol
|
3 MICROMOLAR |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
KBr
Br.K 119.002 g/mol
|
6 MICROMOLAR |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Co(NO3)2.6H2O
Co.6H2O.2NO3 291.031 g/mol
|
3 MICROMOLAR |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
(NH4)6Mo7O24.4H2O
2H4N.MoO4 196.014 g/mol
|
0.4 MICROMOLAR |
CHEBI:91249
ammonium molybdate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 | VSO5.5H2O | 0.6 MICROMOLAR | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 22 | Cr(NO3)3.9H2O | 0.6 MICROMOLAR | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 23 | SeO2 | 3 MICROMOLAR | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 24 | Cd(NO3).4H2O | 3 MICROMOLAR | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 25 | 50mM NaH2PO4 | 100 G_PER_L | ||
| 26 | 50mM Na2HPO4 | 100 G_PER_L | ||
| 27 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
6 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
3.55 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 29 | 0.1N HCl | 40 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 30 |
FeCl3
Cl3Fe 162.204 g/mol
|
1.08 G_PER_L |
CHEBI:30808
iron trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 | 0.1N NaOH | 40 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 32 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
1.488 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
Solutions
FeCl3 solution
Dose: 40 G_PER_L
Na2-EDTA solution
Dose: 40 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pcr_s11_medium (CultureMech:008849)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.236990Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2262 |
| 2026-01-28T19:48:25.551076Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:42.303734+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:12.324567Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:00.745540Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008849 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.425605+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PCR-S11 medium" to "pcr_s11_medium" for consistent matching |
| 2026-05-16T09:04:28.462848+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:40.989798+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.910453+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.307461+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7f448f452bbee855d1c18c6e3ca2860f041c7408c06143f8c45f1e5bb9f614d1 |