Overview
- External ontology
- TOGO:M2274 — Chocolate agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2274 Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008860["chocolate_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_008860 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Hemin["Hemin"]
CultureMech_008860 --> ing1_Hemin
CHEBI_50385["CHEBI:50385"]
ing1_Hemin -.-> CHEBI_50385
ing2_Menadione["Menadione"]
CultureMech_008860 --> ing2_Menadione
CHEBI_28869["CHEBI:28869"]
ing2_Menadione -.-> CHEBI_28869
ing3_Sheep_blood["Sheep blood"]
CultureMech_008860 --> ing3_Sheep_blood
ing4_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_008860 --> ing4_Pancreatic_digest_of_casein
ing5_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_008860 --> ing5_Peptic_digest_of_soybean_meal
ing6_Glucose["Glucose"]
CultureMech_008860 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Sodium_chloride["Sodium chloride"]
CultureMech_008860 --> ing7_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing7_Sodium_chloride -.-> CHEBI_26710
ing8_Dipotassium_phosphate["Dipotassium phosphate"]
CultureMech_008860 --> ing8_Dipotassium_phosphate
CHEBI_131527["CHEBI:131527"]
ing8_Dipotassium_phosphate -.-> CHEBI_131527
ing9_Agar["Agar"]
CultureMech_008860 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_CO2["CO2"]
CultureMech_008860 --> ing10_CO2
CHEBI_16526["CHEBI:16526"]
ing10_CO2 -.-> CHEBI_16526
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
5 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Defined component, Organic compound, Simple component |
| 3 |
Menadione
C11H8O2 172.183 g/mol
|
0.2 G_PER_L |
CHEBI:28869
menadione
|
Role: Vitamin; Properties: Defined component, Organic compound, Simple component |
| 4 | Sheep blood | 10 PERCENT_W_V | Role: Nutrient source; Properties: Undefined component | |
| 5 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 6 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 7 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 8 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 9 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 10 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 11 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2274_Chocolate_agar (CultureMech:008860)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.253760Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2274 |
| 2026-01-28T19:48:14.141469Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2 |
| 2026-03-13T06:20:00.808561Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008860 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.494636+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chocolate agar" to "chocolate_agar" for consistent matching |
| 2026-05-16T09:03:12.731353+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.909528+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:10.840956+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1637869dd8f5603e091c62995f400a7eb921608c157bdf40bda0b8bd102dae9e |