Overview
- External ontology
- TOGO:M2279 — Singulisphaera Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2279 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1144.pdf
Composition
flowchart LR
CultureMech_008864["singulisphaera_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008864 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008864 --> ing1_Yeast_extract
ing2_KH2PO4["KH2PO4"]
CultureMech_008864 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_N_acetylglucosamine["N-acetylglucosamine"]
CultureMech_008864 --> ing3_N_acetylglucosamine
CHEBI_59640["CHEBI:59640"]
ing3_N_acetylglucosamine -.-> CHEBI_59640
ing4_Na_ampicillin__may_be_omitted_for_pure_cultures_["Na-ampicillin (may be omitted for pure cultures)"]
CultureMech_008864 --> ing4_Na_ampicillin__may_be_omitted_for_pure_cultures_
ing5_Agar_agar__Difco_["Agar-agar (Difco)"]
CultureMech_008864 --> ing5_Agar_agar__Difco_
ing6_Peptone["Peptone"]
CultureMech_008864 --> ing6_Peptone
ing7_Hutners_basal_salts__see_below_["Hutners basal salts (see below)"]
CultureMech_008864 --> ing7_Hutners_basal_salts__see_below_
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008864 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_008864 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_008864 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
CultureMech_008864 --> ing11_Nitrilotriacetic_acid__NTA_
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
ing12__NH4_MoO7O24_x_4H2O["(NH4)MoO7O24 x 4H2O"]
CultureMech_008864 --> ing12__NH4_MoO7O24_x_4H2O
ing13__Metals_44___see_below_["“Metals 44” (see below)"]
CultureMech_008864 --> ing13__Metals_44___see_below_
ing14_KOH["KOH"]
CultureMech_008864 --> ing14_KOH
CHEBI_32035["CHEBI:32035"]
ing14_KOH -.-> CHEBI_32035
ing15_NaOH["NaOH"]
CultureMech_008864 --> ing15_NaOH
CHEBI_32145["CHEBI:32145"]
ing15_NaOH -.-> CHEBI_32145
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_008864 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_008864 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_008864 --> ing18_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing18_MnSO4_x_H2O -.-> CHEBI_86364
ing19_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_008864 --> ing19_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing19_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
ing20_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_008864 --> ing20_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing20_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing21_Na_EDTA["Na-EDTA"]
CultureMech_008864 --> ing21_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing21_Na_EDTA -.-> CHEBI_64734
ing22_H2SO4["H2SO4"]
CultureMech_008864 --> ing22_H2SO4
CHEBI_26836["CHEBI:26836"]
ing22_H2SO4 -.-> CHEBI_26836
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2950.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1000.0, 950.0, 1000.0] |
| 2 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 | N-acetylglucosamine | 1 G_PER_L |
CHEBI:59640
N-acetylglucosamine
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 5 | Na-ampicillin (may be omitted for pure cultures) | 0.2 G_PER_L | Role: Antimicrobial agent; Properties: Defined component, Simple component | |
| 6 | Agar-agar (Difco) | 18 G_PER_L | Role: Solidifying component | |
| 7 | Peptone | 0.1 G_PER_L | ||
| 8 | Hutners basal salts (see below) | 20 G_PER_L | ||
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
29.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.34 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
599.0 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 99.0, 500.0] |
| 12 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 13 | (NH4)MoO7O24 x 4H2O | 9.25 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 14 | “Metals 44” (see below) | 50 G_PER_L | ||
| 15 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 16 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1095 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
39.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
154 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
24.8 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
17.7 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
250 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 23 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2279_Singulisphaera_Medium (CultureMech:008864)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.258615Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2279 |
| 2026-01-28T19:48:08.117466Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: NaOH; Added default concentration for ingredient: H2SO4 |
| 2026-03-13T03:08:40.013710Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:00.833021Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008864 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.519732+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Singulisphaera Medium" to "singulisphaera_medium" for consistent matching |
| 2026-05-16T09:03:12.743984+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.922085+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:31.920235+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:25.844426+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.739007+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:10.855402+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6a3ec18781e61cd2af8e4bbf3caf99e542101d5b2e7106c73ff6118122864717 |