Overview
- External ontology
- TOGO:M2282 — OS Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2282 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium887.pdf
Composition
flowchart LR
CultureMech_008868["os_medium"]
ing0_NaCl["NaCl"]
CultureMech_008868 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_008868 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_008868 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_H3BO3["H3BO3"]
CultureMech_008868 --> ing3_H3BO3
CHEBI_33118["CHEBI:33118"]
ing3_H3BO3 -.-> CHEBI_33118
ing4_KCl["KCl"]
CultureMech_008868 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaHCO3["NaHCO3"]
CultureMech_008868 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_008868 --> ing6_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
ing7_NaNO3["NaNO3"]
CultureMech_008868 --> ing7_NaNO3
CHEBI_63005["CHEBI:63005"]
ing7_NaNO3 -.-> CHEBI_63005
ing8_Na2SO4["Na2SO4"]
CultureMech_008868 --> ing8_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing8_Na2SO4 -.-> CHEBI_32149
ing9_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_008868 --> ing9_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing9_MnSO4_x_4_H2O -.-> CHEBI_86358
ing10_H2O["H2O"]
CultureMech_008868 --> ing10_H2O
CHEBI_15377["CHEBI:15377"]
ing10_H2O -.-> CHEBI_15377
ing11_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_008868 --> ing11_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing11_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing12_H2SO4["H2SO4"]
CultureMech_008868 --> ing12_H2SO4
CHEBI_26836["CHEBI:26836"]
ing12_H2SO4 -.-> CHEBI_26836
ing13_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008868 --> ing13_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing13_Carbon_dioxide_gas -.-> CHEBI_16526
ing14_Nitrogen_gas["Nitrogen gas"]
CultureMech_008868 --> ing14_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing14_Nitrogen_gas -.-> CHEBI_17997
ing15_Oxygen_gas["Oxygen gas"]
CultureMech_008868 --> ing15_Oxygen_gas
ing16_Hydrogen_gas["Hydrogen gas"]
CultureMech_008868 --> ing16_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing16_Hydrogen_gas -.-> CHEBI_18276
ing17_Distilled_water["Distilled water"]
CultureMech_008868 --> ing17_Distilled_water
ing17_Distilled_water -.-> CHEBI_15377
ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008868 --> ing18_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_008868 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_008868 --> ing20_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_008868 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_008868 --> ing22_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing23_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_008868 --> ing23_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing23_CuSO4_x_5_H2O -.-> CHEBI_31440
ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_008868 --> ing24_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing25_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_008868 --> ing25_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing25_CoSO4_x_7_H2O -.-> CHEBI_91244
ing26_KAl_SO4_2_x_12_H2O["KAl(SO4)2 x 12 H2O"]
CultureMech_008868 --> ing26_KAl_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing26_KAl_SO4_2_x_12_H2O -.-> CHEBI_86465
ing27_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_008868 --> ing27_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing27_Nitrilotriacetic_acid -.-> CHEBI_44557
ing28_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_008868 --> ing28_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing28_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing29_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_008868 --> ing29_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing29_MnSO4_x_H2O -.-> CHEBI_86364
CultureMech_008868 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
257.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 256.0, 1.0] |
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.9 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.8, 0.1] |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.7 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
H3BO3
H3BO3 61.833 g/mol
|
10.31 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 10.3, 0.01] |
| 5 |
KCl
Cl.K 74.551 g/mol
|
15 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.1 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NaNO3
NO3.Na 84.994 g/mol
|
0.3 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2SO4
2Na.O4S 142.043 g/mol
|
23 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.06 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
H2O
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media |
| 12 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 13 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 14 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 15 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 16 | Oxygen gas | variable VARIABLE | Properties: ガス | |
| 17 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
| 18 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
KAl(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 29 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.4 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
Trace element solution
Dose: 10 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2282_OS_Medium (CultureMech:008868)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.269191Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2282 |
| 2026-01-28T19:48:33.431321Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:55.429801+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:26.925900Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:00.888709Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008868 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.580499+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "OS Medium" to "os_medium" for consistent matching |
| 2026-05-16T09:03:12.805062+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:35.984136+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:31.961157+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:25.908594+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.761442+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:10.926436+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bd60193e673387e64edcfc5a84283472023b99377d8dd856adb62f3437a39127 |