persephonella_medium

CultureMech:008870 bacterial COMPLEX LIQUID
Original name: Persephonella Medium

Overview

External ontology
TOGO:M2284 — Persephonella Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M2284 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium996.pdf

Composition

flowchart LR
    CultureMech_008870["persephonella_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008870 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_008870 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_008870 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_008870 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_008870 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_008870 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_KCl["KCl"]
    CultureMech_008870 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_K2HPO4x_3_H2O["K2HPO4x 3 H2O"]
    CultureMech_008870 --> ing7_K2HPO4x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4x_3_H2O -.-> CHEBI_131527
    ing8_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_008870 --> ing8_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing8_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing9_NaOH["NaOH"]
    CultureMech_008870 --> ing9_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing9_NaOH -.-> CHEBI_32145
    ing10_H2SO4["H2SO4"]
    CultureMech_008870 --> ing10_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing10_H2SO4 -.-> CHEBI_26836
    ing11_CO2["CO2"]
    CultureMech_008870 --> ing11_CO2
    CHEBI_16526["CHEBI:16526"]
    ing11_CO2 -.-> CHEBI_16526
    ing12_O2["O2"]
    CultureMech_008870 --> ing12_O2
    ing13_H2["H2"]
    CultureMech_008870 --> ing13_H2
    CHEBI_18276["CHEBI:18276"]
    ing13_H2 -.-> CHEBI_18276
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_008870 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_H3BO3["H3BO3"]
    CultureMech_008870 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_008870 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_008870 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_008870 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_ZnCl2["ZnCl2"]
    CultureMech_008870 --> ing19_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing19_ZnCl2 -.-> CHEBI_49976
    ing20_Na2O4W_x_2_H2O["Na2O4W x 2 H2O"]
    CultureMech_008870 --> ing20_Na2O4W_x_2_H2O
    ing21_Ni2SO4x_6_H2O["Ni2SO4x 6 H2O"]
    CultureMech_008870 --> ing21_Ni2SO4x_6_H2O
    ing22_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
    CultureMech_008870 --> ing22_AlCl3_x_6_H2O
    CHEBI_30115["CHEBI:30115"]
    ing22_AlCl3_x_6_H2O -.-> CHEBI_30115
    ing23_H2SeO3_selenous_acid_["H2SeO3(selenous acid)"]
    CultureMech_008870 --> ing23_H2SeO3_selenous_acid_
    ing24_CuCl["CuCl"]
    CultureMech_008870 --> ing24_CuCl
    CHEBI_53472["CHEBI:53472"]
    ing24_CuCl -.-> CHEBI_53472
    ing25_Na_EDTA_x_2_H2O["Na-EDTA x 2 H2O"]
    CultureMech_008870 --> ing25_Na_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing25_Na_EDTA_x_2_H2O -.-> CHEBI_64758

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
990 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 NaCl
Cl.Na 58.443 g/mol
29 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.4 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.2 G_PER_L CHEBI:31206
ammonium chloride
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
1.36 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 K2HPO4x 3 H2O
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
9 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
2 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
Properties: Defined component, Inorganic compound, Simple component
10 NaOH
HO.Na 39.997 g/mol
2 G_PER_L CHEBI:32145
sodium hydroxide
Properties: Defined component, Inorganic compound, Simple component
11 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
12 CO2
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: ガス
13 O2 variable VARIABLE Properties: ガス
14 H2
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
Properties: ガス
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
10 G_PER_L CHEBI:75213
sodium molybdate dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16 H3BO3
H3BO3 61.833 g/mol
10 G_PER_L CHEBI:33118
boric acid
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
100 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
100 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
150 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
20 ZnCl2
2Cl.Zn 136.296 g/mol
100 G_PER_L CHEBI:49976
zinc dichloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21 Na2O4W x 2 H2O 30 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22 Ni2SO4x 6 H2O 20 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
23 AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
40 G_PER_L CHEBI:30115
aluminium trichloride hexahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
24 H2SeO3(selenous acid) 10 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
25 CuCl
ClCu 98.999 g/mol
20 G_PER_L CHEBI:53472
copper(I) chloride
Role: Mineral source
26 Na-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
500 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
Properties: Simple component, Organic compound, Defined component

Solutions

Trace element solution (see below)

Dose: 10 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.277134Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2284
2026-01-28T19:48:17.642018Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: CO2; Added default concentration for ingredient: O2; Added default concentration for ingredient: H2
2026-03-09T08:04:29.229883+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:00.924002Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008870
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:19.616682+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Persephonella Medium" to "persephonella_medium" for consistent matching
2026-05-16T09:03:12.844525+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:36.023944+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:25.949049+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:48.778617+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:10.971410+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 849ec992af8b1bd5bb1d339558517cb729ae76a44120862e04718a995137e3c0