Overview
- External ontology
- TOGO:M2297 — Acidithiobacillus ferrooxidans Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2297 Original URL: https://www.atcc.org/~/media/4C29EA2E2A0749BB9354D8AC64AA715B.ashx
Composition
flowchart LR
CultureMech_008883["acidithiobacillus_ferrooxidans_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008883 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_K2HPO4["K2HPO4"]
CultureMech_008883 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_008883 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_DI_Water["DI Water"]
CultureMech_008883 --> ing3_DI_Water
ing4_H2SO4["H2SO4"]
CultureMech_008883 --> ing4_H2SO4
CHEBI_26836["CHEBI:26836"]
ing4_H2SO4 -.-> CHEBI_26836
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_008883 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_NaCl["NaCl"]
CultureMech_008883 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_008883 --> ing7_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing8_H3BO3["H3BO3"]
CultureMech_008883 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_008883 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_008883 --> ing10_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_008883 --> ing11_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
ing12_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_008883 --> ing12_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing12_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_008883 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_CaCl2["CaCl2"]
CultureMech_008883 --> ing14_CaCl2
CHEBI_3312["CHEBI:3312"]
ing14_CaCl2 -.-> CHEBI_3312
ing15_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_008883 --> ing15_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing15_MnSO4_x_H2O -.-> CHEBI_86364
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
5.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.0, 3.0] |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.8 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 4 | DI Water | 2000.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 800.0, 200.0, 1000.0] | |
| 5 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
20.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 20.0, 0.1] |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.1 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 15 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 800 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 200 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
*Wolfe's Mineral Solution (see below)
Dose: 5 G_PER_L
Potassium hydroxide solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2297_Acidithiobacillus_ferrooxidans_Medium (CultureMech:008883)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.295580Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2297 |
| 2026-01-28T19:48:14.171866Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: Potassium hydroxide solution |
| 2026-03-09T08:04:23.472226+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:51.735583Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:01.008457Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008883 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.706708+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Acidithiobacillus ferrooxidans Medium" to "acidithiobacillus_ferrooxidans_medium" for consistent matching |
| 2026-05-16T09:03:12.910246+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:36.099796+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.016251+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:26.020076+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.800847+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:11.046541+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f1d1d868982308caba9f94a76325d36ec7a2aa281542fefeecc8c0d05e0850e2 |