Overview
- External ontology
- TOGO:M2301 — R2A Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2301 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830.pdf
Composition
flowchart LR
CultureMech_008888["r2a_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008888 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008888 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008888 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_008888 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Na_pyruvate["Na-pyruvate"]
CultureMech_008888 --> ing4_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing4_Na_pyruvate -.-> CHEBI_50144
ing5_Glucose["Glucose"]
CultureMech_008888 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Soluble_starch["Soluble starch"]
CultureMech_008888 --> ing6_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing6_Soluble_starch -.-> CHEBI_28017
ing7_Agar["Agar"]
CultureMech_008888 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Casamino_acids["Casamino acids"]
CultureMech_008888 --> ing8_Casamino_acids
ing9_Proteose_Peptone__Difco_no__3_["Proteose Peptone (Difco no. 3)"]
CultureMech_008888 --> ing9_Proteose_Peptone__Difco_no__3_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 7 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 9 | Casamino acids | 0.5 G_PER_L | Properties: Undefined component | |
| 10 | Proteose Peptone (Difco no. 3) | 0.5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- TOGO_M2301_R2A_Medium (CultureMech:008888)
- TOGO_M2592_R2A_Medium (CultureMech:009159)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.304427Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2301 |
| 2026-01-28T19:48:12.713451Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:01.058701Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008888 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.759573+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2A Medium" to "r2a_medium" for consistent matching |
| 2026-06-05T07:40:36.151181+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.039977+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:26.073135+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.824179+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:11.113869+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: TOGO_M2301_R2A_Medium, TOGO_M2592_R2A_Medium |
| 2026-08-06T03:05:11.113919+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint be82256a583fdec40cdf7ad38cce6b6bb15c24871b0bf7c8bb2927a8b2842f15 |