Overview
- External ontology
- TOGO:M2309 — Revised Sulfolobus medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2309 Original URL: https://www.atcc.org/~/media/A68382D4E7734492831FFBFABAE0FD2A.ashx
Composition
flowchart LR
CultureMech_008895["revised_sulfolobus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008895 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008895 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008895 --> ing2_Yeast_extract
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008895 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_008895 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008895 --> ing5_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing5_Na2MoO4_2H2O -.-> CHEBI_75213
ing6_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008895 --> ing6_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing6_MnCl2_4H2O -.-> CHEBI_86368
ing7_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008895 --> ing7_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_7H2O -.-> CHEBI_32312
ing8_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008895 --> ing8_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing8_CuCl2_2H2O -.-> CHEBI_86318
ing9__NH4_2SO4["(NH4)2SO4"]
CultureMech_008895 --> ing9__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing9__NH4_2SO4 -.-> CHEBI_62946
ing10_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_008895 --> ing10_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing10_FeCl3_6H2O -.-> CHEBI_86254
ing11_Na2B4O7["Na2B4O7"]
CultureMech_008895 --> ing11_Na2B4O7
CHEBI_38892["CHEBI:38892"]
ing11_Na2B4O7 -.-> CHEBI_38892
ing12_VOSO4_2_H2O["VOSO4·2 H2O"]
CultureMech_008895 --> ing12_VOSO4_2_H2O
CHEBI_87009["CHEBI:87009"]
ing12_VOSO4_2_H2O -.-> CHEBI_87009
ing13_CoSO4["CoSO4"]
CultureMech_008895 --> ing13_CoSO4
CHEBI_53470["CHEBI:53470"]
ing13_CoSO4 -.-> CHEBI_53470
ing14_Tryptone__BD_211705_["Tryptone (BD 211705)"]
CultureMech_008895 --> ing14_Tryptone__BD_211705_
ing15_H2SO4["H2SO4"]
CultureMech_008895 --> ing15_H2SO4
CHEBI_26836["CHEBI:26836"]
ing15_H2SO4 -.-> CHEBI_26836
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.05 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.02 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Na2B4O7
H4B4O9.2Na 237.251 g/mol
|
4.5 G_PER_L |
CHEBI:38892
disodium tetraborate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
VOSO4·2 H2O
2H2O.O4S.OV 199.036 g/mol
|
0.03 G_PER_L |
CHEBI:87009
vanadyl sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CoSO4
Co.O4S 154.996 g/mol
|
0.01 G_PER_L |
CHEBI:53470
cobalt(2+) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 | Tryptone (BD 211705) | 1 G_PER_L | Properties: Undefined component | |
| 16 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- revised_sulfolobus_medium (CultureMech:008895)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.317265Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2309 |
| 2026-01-28T19:48:31.091047Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-13T06:20:01.113501Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008895 |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.816933+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Revised Sulfolobus medium" to "revised_sulfolobus_medium" for consistent matching |
| 2026-05-16T09:04:30.541244+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:44.173181+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:31.072091+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:10.893180+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.680518+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e52e8dd7050023fddf880f6b8753344101e9db88d3b0916fda7bf1904af3a99f |